Influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb(II) ion-selective electrode

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

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Influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb(II) ion-selective electrode. / Joon, Narender Kumar; He, Ning; Wagner, Michal; Cárdenas, Marité; Bobacka, Johan; Lisak, Grzegorz.

In: Electrochimica Acta, Vol. 252, 2017, p. 490-497.

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

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Joon, Narender Kumar ; He, Ning ; Wagner, Michal ; Cárdenas, Marité ; Bobacka, Johan ; Lisak, Grzegorz. / Influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb(II) ion-selective electrode. In: Electrochimica Acta. 2017 ; Vol. 252. pp. 490-497.

Bibtex

@article{375508d9ed634e158a76a79c7050f8a2,
title = "Influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb(II) ion-selective electrode",
abstract = "In this work, the influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb2+-selective electrode (Pb2+-ISE) was studied. The effects of bovine serum albumin (BSA) adsorption at the surface of the ion-selective membrane combined with electrode conditioning in phosphate-buffered saline (PBS) solution was elucidated by potentiometry and electrochemical impedance spectroscopy. The adsorbed BSA at the surface of the Pb2+-ISE slightly lowered the detection limit but did not influence the selectivity of the Pb2+-ISE towards the interfering ions studied (Cu2+, Cd2+). Conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS resulted in a super-Nernstian response which was related to fixation/extraction of Pb2+ in the ion-selective membrane via precipitation of Pb3(PO4)2 by PO43– anions present in PBS. By conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS + 1 mg/ml BSA it was possible to extend the linear response range of the Pb2+-ISE towards lower analyte concentrations. The utilization of this conditioning procedure was validated by determination of Pb2+ concentrations down to ca 20 ppb in aqueous samples by Pb2+-ISEs and by comparing the results with those obtained by ICP-MS.",
keywords = "Bovine serum albumin, Phosphate-buffered saline, Potentiometric sensors, Non-equilibrium potentiometry, Protein adsorption",
author = "Joon, {Narender Kumar} and Ning He and Michal Wagner and Marit{\'e} C{\'a}rdenas and Johan Bobacka and Grzegorz Lisak",
year = "2017",
doi = "10.1016/j.electacta.2017.08.126",
language = "English",
volume = "252",
pages = "490--497",
journal = "Electrochimica Acta",
issn = "0013-4686",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb(II) ion-selective electrode

AU - Joon, Narender Kumar

AU - He, Ning

AU - Wagner, Michal

AU - Cárdenas, Marité

AU - Bobacka, Johan

AU - Lisak, Grzegorz

PY - 2017

Y1 - 2017

N2 - In this work, the influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb2+-selective electrode (Pb2+-ISE) was studied. The effects of bovine serum albumin (BSA) adsorption at the surface of the ion-selective membrane combined with electrode conditioning in phosphate-buffered saline (PBS) solution was elucidated by potentiometry and electrochemical impedance spectroscopy. The adsorbed BSA at the surface of the Pb2+-ISE slightly lowered the detection limit but did not influence the selectivity of the Pb2+-ISE towards the interfering ions studied (Cu2+, Cd2+). Conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS resulted in a super-Nernstian response which was related to fixation/extraction of Pb2+ in the ion-selective membrane via precipitation of Pb3(PO4)2 by PO43– anions present in PBS. By conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS + 1 mg/ml BSA it was possible to extend the linear response range of the Pb2+-ISE towards lower analyte concentrations. The utilization of this conditioning procedure was validated by determination of Pb2+ concentrations down to ca 20 ppb in aqueous samples by Pb2+-ISEs and by comparing the results with those obtained by ICP-MS.

AB - In this work, the influence of phosphate buffer and proteins on the potentiometric response of a polymeric membrane-based solid-contact Pb2+-selective electrode (Pb2+-ISE) was studied. The effects of bovine serum albumin (BSA) adsorption at the surface of the ion-selective membrane combined with electrode conditioning in phosphate-buffered saline (PBS) solution was elucidated by potentiometry and electrochemical impedance spectroscopy. The adsorbed BSA at the surface of the Pb2+-ISE slightly lowered the detection limit but did not influence the selectivity of the Pb2+-ISE towards the interfering ions studied (Cu2+, Cd2+). Conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS resulted in a super-Nernstian response which was related to fixation/extraction of Pb2+ in the ion-selective membrane via precipitation of Pb3(PO4)2 by PO43– anions present in PBS. By conditioning of the Pb2+-ISE in 0.01 mol dm–3 PBS + 1 mg/ml BSA it was possible to extend the linear response range of the Pb2+-ISE towards lower analyte concentrations. The utilization of this conditioning procedure was validated by determination of Pb2+ concentrations down to ca 20 ppb in aqueous samples by Pb2+-ISEs and by comparing the results with those obtained by ICP-MS.

KW - Bovine serum albumin

KW - Phosphate-buffered saline

KW - Potentiometric sensors

KW - Non-equilibrium potentiometry

KW - Protein adsorption

U2 - 10.1016/j.electacta.2017.08.126

DO - 10.1016/j.electacta.2017.08.126

M3 - Journal article

VL - 252

SP - 490

EP - 497

JO - Electrochimica Acta

JF - Electrochimica Acta

SN - 0013-4686

ER -