Influence of ipilimumab on expanded tumour derived T cells from patients with metastatic melanoma

Jon Bjørn, Rikke Birgitte Lyngaa, Rikke Andersen, Lisbet Holmich Rosenkrantz, Sine Reker Hadrup, Marco Donia, Inge Marie Svane

    Research output: Contribution to journalJournal articleResearchpeer-review

    269 Downloads (Pure)


    Introduction: Tumour infiltrating lymphocyte (TIL) based adoptive cell therapy (ACT) is a promising treatment for patients with advanced melanoma. Retrospective studies suggested an association between previous treatment with anti-CTLA-4 antibodies and long term survival after subsequent ACT. Thus, we hypothesized that treatment with anti-CTLA-4 antibodies can induce favourable changes to be detected in TILs.Results: Expanded T cells from Ipilimumab treated patients had a higher proportion of cells expressing CD27, intracellular CTLA-4, TIM-3 and LAG-3. In addition, broader and more frequent T cell responses against common tumour antigens were detected in patients treated with Ipilimumab as compared to anti-CTLA-4 naive patients.Materials and methods: Expanded TILs were obtained from patients with advanced melanoma who had received Ipilimumab in the previous six months, or had not received any type of anti-CTLA-4 antibody. T cell specificity and expression of phenotypic and exhaustion markers were scrutinized as well as functional properties.Conclusions: Ipilimumab may induce tumor-infiltration of T cells of a more naive phenotype expressing markers related to activation or exhaustion. Additionally, Ipilimumab may increase the frequency of T cells recognizing common tumour associated antigens.
    Original languageEnglish
    Issue number16
    Pages (from-to)27062-27074
    Publication statusPublished - 2017

    Bibliographical note

    This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

    Fingerprint Dive into the research topics of 'Influence of ipilimumab on expanded tumour derived T cells from patients with metastatic melanoma'. Together they form a unique fingerprint.

    Cite this