Influence of carbon source on alpha-amylase production by Aspergillus oryzae

Morten Carlsen, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The influence of the carbon source on a-amylase production by Aspergillus oryzae was quantified in carbon-limited chemostat cultures. The following carbon sources were investigated: maltose, maltodextrin (different chain lengths), glucose, fructose, galactose, sucrose, glycerol, mannitol and acetate. A. oryzae did not grow on galactose as the sole carbon source, but galactose was co-metabolized together with glucose. Relative to that on low glucose concentration (below 10 mg/l), productivity was found to be higher during growth on maltose and maltodextrins, whereas it was lower during growth on sucrose, fructose, glycerol, mannitol and acetate. During growth on acetate there was no production of alpha -amylase, whereas addition of small amounts of glucose resulted in alpha -amylase production. A possible induction by alpha -methyl-D-glucoside during growth on glucose was also investigated, but this compound was not found to be a better inducer of alpha -amylase production than glucose. The results strongly indicate that besides acting as a repressor via the CreA protein, glucose acts as an inducer.
    Original languageEnglish
    JournalApplied Microbiology and Biotechnology
    Volume57
    Issue number3
    Pages (from-to)346-349
    ISSN0175-7598
    Publication statusPublished - 2001

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