Induction of oral tolerance with micro-doses of ovomucoid depends on the length of the feeding period

Tanja Kjær, Hanne Frøkiær

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Oral administration of antigen induces antigen-specific immunologic tolerance, which is known to be dose-dependent. We studied the influence of continuous oral administration of nanogram and microgram doses of antigen on oral tolerance induction. Mice were continuously exposed to varying doses (1 ng-1 mg/day) of ovomucoid (OM) for a minimum of 30 days and a maximum of 100 days. It was possible to induce oral tolerance measured as reduced proliferation and antibody production (immunoglobulin (Ig)G(1), IgG(2a), and total Igs) when mice were fed 1 mg of OM/day for 40 or 50 days. It was not possible to induce oral tolerance with daily doses of antigen of 10 mug or less. Feeding of 100 mug OM/day for 40 and 50 days and 1 mg OM/day for 30 days generated tolerization of Th2-dependent responses, but retained an intact response of Th1-dependent antibodies, whereas feeding of 1 mg OM/day for 40 and 50 days resulted in tolerization of both Th1- and Th2-antibody responses. The results presented here suggest that there is a threshold of microgram-doses below which oral tolerance cannot be induced, and that selective suppression of Th2 responses can be achieved by continuous microdose feeding, while an extension of the feeding dose or feeding period tolerizes both Th1- and Th2-dependent responses.
    Original languageEnglish
    JournalScandinavian Journal of Immunology
    Volume55
    Issue number4
    Pages (from-to)359-365
    ISSN0300-9475
    Publication statusPublished - 2002

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