Increasing Oil Bodies in Physcomitrella patens by Overexpressing Oil Body- Associated Proteins

Hansol Bae, Anantha Vithakshana Peramuna, Henrik Toft Simonsen

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Abstract

In bryophytes, reproductive organs contain large amount of oil bodies (OBs), the well-known lipidcontaining structures. OBs in spores are the most prominent and have been extensively studied. They are thought to be formed by budding off the outer layer of the endoplasmic reticulum membrane (ER) due to accumulation of neutral lipids between the leaflets of the phospholipid bilayer. In P. patens, the OBs are abundant in the mature sporophytes or during starvation, but are hardly observed in the vegetative stage. We overexpressed OB associated proteins such as oleosin, seipin, and fibrillin to increase the OB formation in P. patens vegetative tissue. As a result, we confirmed that the number of OBs were significantly increased in the protonemal cells compared to the wild type. These structures could be used to compartmentalize, synthetically made high-value compounds in green cells.
Original languageEnglish
Publication date2016
Publication statusPublished - 2016
EventSustain-ATV Conference 2016: Creating Technology for a Sustainable Society - Technical University of Denmark, Kgs. Lyngby, Denmark
Duration: 30 Nov 201630 Nov 2016
http://www.sustain.dtu.dk/about/sustain-2016

Conference

ConferenceSustain-ATV Conference 2016
LocationTechnical University of Denmark
CountryDenmark
CityKgs. Lyngby
Period30/11/201630/11/2016
Internet address

Bibliographical note

Sustain Abstract B-7

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