In bryophytes, reproductive organs contain large amount of oil bodies (OBs), the well-known lipidcontaining structures. OBs in spores are the most prominent and have been extensively studied. They are thought to be formed by budding off the outer layer of the endoplasmic reticulum membrane (ER) due to accumulation of neutral lipids between the leaflets of the phospholipid bilayer. In P. patens, the OBs are abundant in the mature sporophytes or during starvation, but are hardly observed in the vegetative stage. We overexpressed OB associated proteins such as oleosin, seipin, and fibrillin to increase the OB formation in P. patens vegetative tissue. As a result, we confirmed that the number of OBs were significantly increased in the protonemal cells compared to the wild type. These structures could be used to compartmentalize, synthetically made high-value compounds in green cells.
|Publication status||Published - 2016|
|Event||Sustain-ATV Conference 2016: Creating Technology for a Sustainable Society - Technical University of Denmark, Kgs. Lyngby, Denmark|
Duration: 30 Nov 2016 → 30 Nov 2016
|Conference||Sustain-ATV Conference 2016|
|Location||Technical University of Denmark|
|Period||30/11/2016 → 30/11/2016|