Increasing levels of dietary crystalline methionine affect plasma methionine profiles, ammonia excretion, and the expression of genes related to the hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)

Marine Rolland, Peter Vilhelm Skov, Bodil Katrine Larsen, Jørgen Holm, Pedro Gómez-Requeni, Anne Johanne Tang Dalsgaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Strictly carnivorous fish with high requirements for dietary protein, such as rainbow trout (Oncorhynchus mykiss) are interesting models for studying the role of amino acids as key regulators of intermediary metabolism. Methionine is an essential amino acid for rainbow trout, and works as a signalling factor in different metabolic pathways. The study investigated the effect of increasing dietary methionine intake on the intermediary metabolism in the liver of juvenile rainbow trout. For this purpose, five diets were formulated with increasing methionine levels from 0.60 to 1.29% dry matter. The diets were fed in excess for six weeks before three sampling campaigns carried out successively to elucidate (i) the hepatic expression of selected genes involved in lipid, glucose and amino acid metabolism; (ii) the postprandial ammonia excretion; and (iii) the postprandial plasma methionine concentrations. The transcript levels of enzymes involved in lipid metabolism (fatty acid synthase, glucose 6 phosphate dehydrogenase and carnitine palmitoyl transferase 1 a), gluconeogenesis (fructose-1,6-biphosphatase) and amino acid catabolism (alanine amino transferase and glutamate dehydrogenase) were significantly affected by the increase in dietary methionine. Changes in gene expression reflected to some extent the decrease in ammonia excretion (P=0.022) and in the hepatosomatic index (HSI; P
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology
Volume198
Pages (from-to)91-99
ISSN1096-4959
DOIs
Publication statusPublished - 2016

Keywords

  • Amino acid catabolism
  • Lipid metabolism
  • Gluconeogenesis
  • Crystalline amino acid
  • Hepatosomatic index
  • Hepatic gene expression

Cite this

@article{3fa80723992b4b76b424730b81664f10,
title = "Increasing levels of dietary crystalline methionine affect plasma methionine profiles, ammonia excretion, and the expression of genes related to the hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)",
abstract = "Strictly carnivorous fish with high requirements for dietary protein, such as rainbow trout (Oncorhynchus mykiss) are interesting models for studying the role of amino acids as key regulators of intermediary metabolism. Methionine is an essential amino acid for rainbow trout, and works as a signalling factor in different metabolic pathways. The study investigated the effect of increasing dietary methionine intake on the intermediary metabolism in the liver of juvenile rainbow trout. For this purpose, five diets were formulated with increasing methionine levels from 0.60 to 1.29{\%} dry matter. The diets were fed in excess for six weeks before three sampling campaigns carried out successively to elucidate (i) the hepatic expression of selected genes involved in lipid, glucose and amino acid metabolism; (ii) the postprandial ammonia excretion; and (iii) the postprandial plasma methionine concentrations. The transcript levels of enzymes involved in lipid metabolism (fatty acid synthase, glucose 6 phosphate dehydrogenase and carnitine palmitoyl transferase 1 a), gluconeogenesis (fructose-1,6-biphosphatase) and amino acid catabolism (alanine amino transferase and glutamate dehydrogenase) were significantly affected by the increase in dietary methionine. Changes in gene expression reflected to some extent the decrease in ammonia excretion (P=0.022) and in the hepatosomatic index (HSI; P",
keywords = "Amino acid catabolism, Lipid metabolism, Gluconeogenesis, Crystalline amino acid, Hepatosomatic index, Hepatic gene expression",
author = "Marine Rolland and Skov, {Peter Vilhelm} and Larsen, {Bodil Katrine} and J{\o}rgen Holm and Pedro G{\'o}mez-Requeni and Dalsgaard, {Anne Johanne Tang}",
year = "2016",
doi = "10.1016/j.cbpb.2016.04.006",
language = "English",
volume = "198",
pages = "91--99",
journal = "Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology",
issn = "1096-4959",
publisher = "Elsevier",

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TY - JOUR

T1 - Increasing levels of dietary crystalline methionine affect plasma methionine profiles, ammonia excretion, and the expression of genes related to the hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)

AU - Rolland, Marine

AU - Skov, Peter Vilhelm

AU - Larsen, Bodil Katrine

AU - Holm, Jørgen

AU - Gómez-Requeni, Pedro

AU - Dalsgaard, Anne Johanne Tang

PY - 2016

Y1 - 2016

N2 - Strictly carnivorous fish with high requirements for dietary protein, such as rainbow trout (Oncorhynchus mykiss) are interesting models for studying the role of amino acids as key regulators of intermediary metabolism. Methionine is an essential amino acid for rainbow trout, and works as a signalling factor in different metabolic pathways. The study investigated the effect of increasing dietary methionine intake on the intermediary metabolism in the liver of juvenile rainbow trout. For this purpose, five diets were formulated with increasing methionine levels from 0.60 to 1.29% dry matter. The diets were fed in excess for six weeks before three sampling campaigns carried out successively to elucidate (i) the hepatic expression of selected genes involved in lipid, glucose and amino acid metabolism; (ii) the postprandial ammonia excretion; and (iii) the postprandial plasma methionine concentrations. The transcript levels of enzymes involved in lipid metabolism (fatty acid synthase, glucose 6 phosphate dehydrogenase and carnitine palmitoyl transferase 1 a), gluconeogenesis (fructose-1,6-biphosphatase) and amino acid catabolism (alanine amino transferase and glutamate dehydrogenase) were significantly affected by the increase in dietary methionine. Changes in gene expression reflected to some extent the decrease in ammonia excretion (P=0.022) and in the hepatosomatic index (HSI; P

AB - Strictly carnivorous fish with high requirements for dietary protein, such as rainbow trout (Oncorhynchus mykiss) are interesting models for studying the role of amino acids as key regulators of intermediary metabolism. Methionine is an essential amino acid for rainbow trout, and works as a signalling factor in different metabolic pathways. The study investigated the effect of increasing dietary methionine intake on the intermediary metabolism in the liver of juvenile rainbow trout. For this purpose, five diets were formulated with increasing methionine levels from 0.60 to 1.29% dry matter. The diets were fed in excess for six weeks before three sampling campaigns carried out successively to elucidate (i) the hepatic expression of selected genes involved in lipid, glucose and amino acid metabolism; (ii) the postprandial ammonia excretion; and (iii) the postprandial plasma methionine concentrations. The transcript levels of enzymes involved in lipid metabolism (fatty acid synthase, glucose 6 phosphate dehydrogenase and carnitine palmitoyl transferase 1 a), gluconeogenesis (fructose-1,6-biphosphatase) and amino acid catabolism (alanine amino transferase and glutamate dehydrogenase) were significantly affected by the increase in dietary methionine. Changes in gene expression reflected to some extent the decrease in ammonia excretion (P=0.022) and in the hepatosomatic index (HSI; P

KW - Amino acid catabolism

KW - Lipid metabolism

KW - Gluconeogenesis

KW - Crystalline amino acid

KW - Hepatosomatic index

KW - Hepatic gene expression

U2 - 10.1016/j.cbpb.2016.04.006

DO - 10.1016/j.cbpb.2016.04.006

M3 - Journal article

VL - 198

SP - 91

EP - 99

JO - Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology

JF - Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology

SN - 1096-4959

ER -