Increasing ethanol productivity during xylose fermentation by cell recycling of recombinant Saccharomyces cerevisiae

Christophe Francois Aime Roca, Lisbeth Olsson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The influence of cell recycling of xylose-fermenting Saccharomyces cerevisiae TMB3001 was investigated during continuous cultivation on a xylose-glucose mixture. By using cell recycling at the dilution rate (D) of 0.05 h(-1), the cell-mass concentration could be increased from 2.2 g l(-1) to 22 g l(-1). Consequently, the volumetric ethanol productivity increased ten-fold, from 0.5 g l(-1) h(-1) to 5.35 g l(-1) h(-1). By increasing the biomass concentration, the xylose consumption rate increased from 0.75 g xylose l(-1) h(-1) without recycling to 1.9 g l(-1) h(-1) with recycling. The specific ethanol productivity was in the range of 0.23-0.26 g g(-1) h(-1) with or without cell recycling, showing that an increased cell-mass concentration did not influence the efficiency of the yeast.
    Original languageEnglish
    JournalApplied Microbiology and Biotechnology
    Volume60
    Issue number5
    Pages (from-to)560-563
    ISSN0175-7598
    Publication statusPublished - 2003

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