Increased amount of Bifidobacterium thermacidophilum and Megasphaera elsdenii in the colonic microbiota of pigs fed a swine dysentery preventive diet containing chicory roots and sweet lupine

Lars Mølbak, L.E. Thomsen, Tim Kåre Jensen, K.E.B. Knudsen, Mette Boye

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Aims: To investigate which specific bacterial species that were stimulated or inhibited in the proximal colon of pigs when a fructan-rich diet was compared with a diet that contained resistant carbohydrates. The study focussed especially on Bifidobacterial species by using a noncultureable approach. Methods and Results: Terminal restriction fragment length polymorphism (T-RFLP) was used to describe differences in the total colonic microbiota as well as in the populations of Bifidobacterium spp. in pigs fed with a fructan-rich diet and a diet containing resistant carbohydrates. The fructan-rich diet has previously been shown to prevent swine dysentery caused by Brachyspira hyodysenteriae. The T-RFLP profiling, 16S rRNA gene cloning and in situ hybridization showed that the pigs fed with the fructan-rich diet had a higher proportion of Bifidobacterium thermacidophilum subsp. porcinum and Megasphaera elsdenii. Conclusions: These findings suggested that the bacterial fructan fermentation occurring in the porcine colon might be cross-feeding of lactate produced by B. thermacidophilum and used by M. elsdenii. Significance and Impact of the Study: B. thermacidophilum and M. elsdenii may be the course of the inhibition of the pathogenic bacteria Brach. hyodysenteriae in colon of pigs when they are fed fructan-rich diets.
    Original languageEnglish
    JournalJournal of Applied Microbiology
    Volume103
    Issue number5
    Pages (from-to)1853-1867
    ISSN1364-5072
    DOIs
    Publication statusPublished - 2007

    Keywords

    • intestinal
    • bifidobacterium
    • inulin
    • fructan
    • chicory
    • T-RFLP
    • prebiotic
    • pig

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