Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B

Aikaterini Argyraki, Merete Markvart, Lars Bjørndal, Thomas Bjarnsholt, Paul Michael Petersen

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Abstract

The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological
replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms
provides perspectives for the treatment of infectious diseases
Original languageEnglish
Article number65004
JournalJournal of Biomedical Optics
Volume22
Issue number6
Number of pages6
ISSN1083-3668
DOIs
Publication statusPublished - 2017

Keywords

  • Disinfection
  • Ultraviolet light
  • Ultraviolet C
  • Ultraviolet B
  • Light-emitting diodes
  • Pseudomonas aeruginosa

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