In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

Sara Zanivan; Alexander Meves; Kristina Behrendt; Erwin Schoof; Lisa J. Neilson; Jürgen Cox; Hao R. Tang; Gabriela Kalna; Janine H. van Ree; Jan M. van Deursen; Carol S. Trempus; Laura M. Machesky; Rune Linding; Sara A. Wickström; Reinhard Fässler; Matthias Mann

doi:10.1016/j.celrep.2013.01.003

In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

Sara Zanivan, Alexander Meves, Kristina Behrendt, Erwin Schoof, Lisa J. Neilson, Jürgen Cox, Hao R. Tang, Gabriela Kalna, Janine H. van Ree, Jan M. van Deursen, Carol S. Trempus, Laura M. Machesky, Rune Linding, Sara A. Wickström, Reinhard Fässler, Matthias Mann

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Abstract

SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.

Original language	English
Journal	Cell Reports
Volume	3
Issue number	2
Pages (from-to)	552-566
DOIs	https://doi.org/10.1016/j.celrep.2013.01.003
Publication status	Published - 2013

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Zanivan, S., Meves, A., Behrendt, K., Schoof, E., Neilson, L. J., Cox, J., Tang, H. R., Kalna, G., van Ree, J. H., van Deursen, J. M., Trempus, C. S., Machesky, L. M., Linding, R., Wickström, S. A., Fässler, R., & Mann, M. (2013). In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis. Cell Reports, 3(2), 552-566. https://doi.org/10.1016/j.celrep.2013.01.003

@article{683fafb6351640d4921e9caa22d341d2,

title = "In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis",

abstract = "SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.",

author = "Sara Zanivan and Alexander Meves and Kristina Behrendt and Erwin Schoof and Neilson, {Lisa J.} and J{\"u}rgen Cox and Tang, {Hao R.} and Gabriela Kalna and {van Ree}, {Janine H.} and {van Deursen}, {Jan M.} and Trempus, {Carol S.} and Machesky, {Laura M.} and Rune Linding and Wickstr{\"o}m, {Sara A.} and Reinhard F{\"a}ssler and Matthias Mann",

year = "2013",

doi = "10.1016/j.celrep.2013.01.003",

language = "English",

volume = "3",

pages = "552--566",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "2",

}

Zanivan, S, Meves, A, Behrendt, K, Schoof, E, Neilson, LJ, Cox, J, Tang, HR, Kalna, G, van Ree, JH, van Deursen, JM, Trempus, CS, Machesky, LM, Linding, R, Wickström, SA, Fässler, R & Mann, M 2013, 'In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis', Cell Reports, vol. 3, no. 2, pp. 552-566. https://doi.org/10.1016/j.celrep.2013.01.003

TY - JOUR

T1 - In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

AU - Zanivan, Sara

AU - Meves, Alexander

AU - Behrendt, Kristina

AU - Schoof, Erwin

AU - Neilson, Lisa J.

AU - Cox, Jürgen

AU - Tang, Hao R.

AU - Kalna, Gabriela

AU - van Ree, Janine H.

AU - van Deursen, Jan M.

AU - Trempus, Carol S.

AU - Machesky, Laura M.

AU - Linding, Rune

AU - Wickström, Sara A.

AU - Fässler, Reinhard

AU - Mann, Matthias

PY - 2013

Y1 - 2013

N2 - SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.

AB - SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.

U2 - 10.1016/j.celrep.2013.01.003

DO - 10.1016/j.celrep.2013.01.003

M3 - Journal article

C2 - 23375375

SN - 2211-1247

VL - 3

SP - 552

EP - 566

JO - Cell Reports

JF - Cell Reports

IS - 2

ER -

In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

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