@inbook{42b0a560db154d47b73503907356005d,
title = "In vivo and in situ replication labeling methods for super-resolution structured illumination microscopy of chromosome territories and chromatin domains",
abstract = "Recent advances in super-resolution microscopy enable the study of subchromosomal chromatin organization in single cells with unprecedented detail. Here we describe refined methods for pulse-chase replication labeling of individual chromosome territories (CTs) and replication domain units in mammalian cell nuclei, with specific focus on their application to three-dimensional structured illumination microscopy (3D-SIM). We provide detailed protocols for highly efficient electroporation-based delivery or scratch loading of cell impermeable fluorescent nucleotides for live cell studies. Furthermore we describe the application of (2′S)-2′-deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) for the in situ detection of segregated chromosome territories with minimized cytotoxic side effects.",
keywords = "Chromatin, Chromosome territories, F-ara-EdU, Replication domains, Replication labeling, Structured illumination microscopy, Super-resolution imaging",
author = "Ezequiel Miron and Cassandravictoria Innocent and Sophia Heyde and Lothar Schermelleh",
year = "2016",
doi = "10.1007/978-1-4939-3631-1_10",
language = "English",
volume = "1431",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "127--140",
booktitle = "Methods in Molecular Biology",
address = "United States",
}