In vivo and 3D visualization of coronary artery development by optical coherence tomography - art. no. 662709

Lars Thrane, K. Norozi, J. Männer, F. Pedersen, S. Mottl-Link, H. Engelbrecht-Larsen, Peter E. Andersen, A. Wessel, T.M. Yelbuz

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


    One of the most critical but poorly understood processes during cardiovascular development is the establishment of a functioning coronary artery (CA) system. Due to the lack of suitable imaging technologies, it is currently impossible to visualize this complex dynamic process on living human embryos. Furthermore, due to methodological limitations, this intriguing process has not been unveiled in living animal embryos, too. We present here, to the best of our knowledge, the first in vivo images of developing CAs obtained from the hearts of chick embryos grown in shell-less cultures. The in vivo images were generated by optical coherence tomography (OCT). The OCT system used in this study is a mobile fiber-based time-domain real-time OCT system operating with a center wavelength of 1330 nm, an A-scan rate of 4 kHz, and a typical frame rate of 8 frames/s. The axial resolution is 17 mu m (in tissue), and the lateral resolution is 30 mu m. The OCT system is optimized for in vivo chick heart visualization and enables OCT movie recording with 8 frames/s, full-automatic 3D OCT scanning, and blood flow visualization, i.e., Doppler OCT imaging. Using this OCT system, we generated in vivo OCT recordings of chick embryo hearts to study the process of connection of the future right coronary artery (RCA) to the aorta. Recordings were made at three critical stages during development: day 8 (no clear connection yet), day 9 (established connection of RCA with the aorta with clear blood flow) and day 10 (further remodeling of the established RCA).
    Original languageEnglish
    Title of host publicationOptical coherence tomography and coherence techniques 3
    EditorsP.E. Andersen, Z. Chen
    Number of pages5
    Place of PublicationBellingham
    PublisherInternational Society for Optical Engineering
    Publication date2007
    ISBN (Print)08-19-46771-3
    Publication statusPublished - 2007
    EventEuropean Conferences on Biomedical Optics 2007 - International Conference Centre Munich, Munich, Germany
    Duration: 17 Jun 200721 Jun 2007


    ConferenceEuropean Conferences on Biomedical Optics 2007
    LocationInternational Conference Centre Munich
    SeriesSPIE Proceedings Series, 6627


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