In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.

Louise Kristine Vigsnæs, Hiroyuki Nakai, Lene Hemmingsen, Joakim Mark Andersen, Sampo J Lahtinen, Louise Enggaard Rasmussen, Maher Abou Hachem, Bent O. Petersen, Jens Øllgaard Duus, Anne S. Meyer, Tine Rask Licht, Birte Svensson

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Abstract

The present study aimed at examining oligosaccharides (OS) for potential stimulation of probiotic bacteria. Nineteen structurally well-defined candidate OS covering groups of β-glucosides, α-glucosides and α-galactosides with degree of polymerization 2-4 were prepared in >100 mg amounts by chemoenzymatic synthesis (i.e. reverse phosphorolysis or transglycosylation). Fourteen of the OS are not naturally occurring and five (β-d-glucosyl-fructose, β-d-glucosyl-xylitol, α-glucosyl-(1,4)-d-mannose, α-glucosyl-(1,4)-d-xylose; α-glucosyl-(1,4)-l-fucose) have recently been synthesized for the first time. These OS have not been previously tested for effects of bacterial growth and here the ability of all 19 OS to support growth of four gastrointestinal bacteria: three probiotic bacteria Bifidobacterium lactis, Bifidobacterium longum, and Lactobacillus acidophilus, and one commensal bacterium, Bacteroides vulgatus has been evaluated in monocultures. The disaccharides β-d-glucosyl-xylitol and β-d-glucosyl-(1,4)-xylose noticeably stimulated growth yields of L. acidophilus NCFM, and additionally, β-d-glucosyl-(1,4)-xylose stimulated B. longum Bl-05. α-Glucosyl-(1,4)-glucosamine and α-glucosyl-(1,4)-N-acetyl-glucosamine enhanced the growth rate of B. animalis subsp. lactis and B. longum Bl-05, whereas L. acidophilus NCFM and Bac. vulgatus did not grow on these OS. α-Galactosyl-(1,6)-α-galactosyl-(1,6)-glucose advanced the growth rate of B. animalis subsp. lactis and L. acidophilus NCFM. Thus several of the structurally well-defined OS supported growth of beneficial gut bacteria. This reflects a broad specificity of their sugar transporters for OS, including specificity for non-naturally occurring OS, hence showing promise for design of novel prebiotics.
Original languageEnglish
JournalFood & Function
Volume4
Issue number5
Pages (from-to)784-793
ISSN2042-6496
DOIs
Publication statusPublished - 2013

Cite this

Vigsnæs, L. K., Nakai, H., Hemmingsen, L., Andersen, J. M., Lahtinen, S. J., Rasmussen, L. E., ... Svensson, B. (2013). In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis. Food & Function, 4(5), 784-793. https://doi.org/10.1039/C3FO30357H
Vigsnæs, Louise Kristine ; Nakai, Hiroyuki ; Hemmingsen, Lene ; Andersen, Joakim Mark ; Lahtinen, Sampo J ; Rasmussen, Louise Enggaard ; Abou Hachem, Maher ; Petersen, Bent O. ; Duus, Jens Øllgaard ; Meyer, Anne S. ; Licht, Tine Rask ; Svensson, Birte. / In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis. In: Food & Function. 2013 ; Vol. 4, No. 5. pp. 784-793.
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title = "In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.",
abstract = "The present study aimed at examining oligosaccharides (OS) for potential stimulation of probiotic bacteria. Nineteen structurally well-defined candidate OS covering groups of β-glucosides, α-glucosides and α-galactosides with degree of polymerization 2-4 were prepared in >100 mg amounts by chemoenzymatic synthesis (i.e. reverse phosphorolysis or transglycosylation). Fourteen of the OS are not naturally occurring and five (β-d-glucosyl-fructose, β-d-glucosyl-xylitol, α-glucosyl-(1,4)-d-mannose, α-glucosyl-(1,4)-d-xylose; α-glucosyl-(1,4)-l-fucose) have recently been synthesized for the first time. These OS have not been previously tested for effects of bacterial growth and here the ability of all 19 OS to support growth of four gastrointestinal bacteria: three probiotic bacteria Bifidobacterium lactis, Bifidobacterium longum, and Lactobacillus acidophilus, and one commensal bacterium, Bacteroides vulgatus has been evaluated in monocultures. The disaccharides β-d-glucosyl-xylitol and β-d-glucosyl-(1,4)-xylose noticeably stimulated growth yields of L. acidophilus NCFM, and additionally, β-d-glucosyl-(1,4)-xylose stimulated B. longum Bl-05. α-Glucosyl-(1,4)-glucosamine and α-glucosyl-(1,4)-N-acetyl-glucosamine enhanced the growth rate of B. animalis subsp. lactis and B. longum Bl-05, whereas L. acidophilus NCFM and Bac. vulgatus did not grow on these OS. α-Galactosyl-(1,6)-α-galactosyl-(1,6)-glucose advanced the growth rate of B. animalis subsp. lactis and L. acidophilus NCFM. Thus several of the structurally well-defined OS supported growth of beneficial gut bacteria. This reflects a broad specificity of their sugar transporters for OS, including specificity for non-naturally occurring OS, hence showing promise for design of novel prebiotics.",
author = "Vigsn{\ae}s, {Louise Kristine} and Hiroyuki Nakai and Lene Hemmingsen and Andersen, {Joakim Mark} and Lahtinen, {Sampo J} and Rasmussen, {Louise Enggaard} and {Abou Hachem}, Maher and Petersen, {Bent O.} and Duus, {Jens {\O}llgaard} and Meyer, {Anne S.} and Licht, {Tine Rask} and Birte Svensson",
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In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis. / Vigsnæs, Louise Kristine; Nakai, Hiroyuki; Hemmingsen, Lene; Andersen, Joakim Mark; Lahtinen, Sampo J; Rasmussen, Louise Enggaard; Abou Hachem, Maher ; Petersen, Bent O.; Duus, Jens Øllgaard; Meyer, Anne S.; Licht, Tine Rask; Svensson, Birte.

In: Food & Function, Vol. 4, No. 5, 2013, p. 784-793.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.

AU - Vigsnæs, Louise Kristine

AU - Nakai, Hiroyuki

AU - Hemmingsen, Lene

AU - Andersen, Joakim Mark

AU - Lahtinen, Sampo J

AU - Rasmussen, Louise Enggaard

AU - Abou Hachem, Maher

AU - Petersen, Bent O.

AU - Duus, Jens Øllgaard

AU - Meyer, Anne S.

AU - Licht, Tine Rask

AU - Svensson, Birte

PY - 2013

Y1 - 2013

N2 - The present study aimed at examining oligosaccharides (OS) for potential stimulation of probiotic bacteria. Nineteen structurally well-defined candidate OS covering groups of β-glucosides, α-glucosides and α-galactosides with degree of polymerization 2-4 were prepared in >100 mg amounts by chemoenzymatic synthesis (i.e. reverse phosphorolysis or transglycosylation). Fourteen of the OS are not naturally occurring and five (β-d-glucosyl-fructose, β-d-glucosyl-xylitol, α-glucosyl-(1,4)-d-mannose, α-glucosyl-(1,4)-d-xylose; α-glucosyl-(1,4)-l-fucose) have recently been synthesized for the first time. These OS have not been previously tested for effects of bacterial growth and here the ability of all 19 OS to support growth of four gastrointestinal bacteria: three probiotic bacteria Bifidobacterium lactis, Bifidobacterium longum, and Lactobacillus acidophilus, and one commensal bacterium, Bacteroides vulgatus has been evaluated in monocultures. The disaccharides β-d-glucosyl-xylitol and β-d-glucosyl-(1,4)-xylose noticeably stimulated growth yields of L. acidophilus NCFM, and additionally, β-d-glucosyl-(1,4)-xylose stimulated B. longum Bl-05. α-Glucosyl-(1,4)-glucosamine and α-glucosyl-(1,4)-N-acetyl-glucosamine enhanced the growth rate of B. animalis subsp. lactis and B. longum Bl-05, whereas L. acidophilus NCFM and Bac. vulgatus did not grow on these OS. α-Galactosyl-(1,6)-α-galactosyl-(1,6)-glucose advanced the growth rate of B. animalis subsp. lactis and L. acidophilus NCFM. Thus several of the structurally well-defined OS supported growth of beneficial gut bacteria. This reflects a broad specificity of their sugar transporters for OS, including specificity for non-naturally occurring OS, hence showing promise for design of novel prebiotics.

AB - The present study aimed at examining oligosaccharides (OS) for potential stimulation of probiotic bacteria. Nineteen structurally well-defined candidate OS covering groups of β-glucosides, α-glucosides and α-galactosides with degree of polymerization 2-4 were prepared in >100 mg amounts by chemoenzymatic synthesis (i.e. reverse phosphorolysis or transglycosylation). Fourteen of the OS are not naturally occurring and five (β-d-glucosyl-fructose, β-d-glucosyl-xylitol, α-glucosyl-(1,4)-d-mannose, α-glucosyl-(1,4)-d-xylose; α-glucosyl-(1,4)-l-fucose) have recently been synthesized for the first time. These OS have not been previously tested for effects of bacterial growth and here the ability of all 19 OS to support growth of four gastrointestinal bacteria: three probiotic bacteria Bifidobacterium lactis, Bifidobacterium longum, and Lactobacillus acidophilus, and one commensal bacterium, Bacteroides vulgatus has been evaluated in monocultures. The disaccharides β-d-glucosyl-xylitol and β-d-glucosyl-(1,4)-xylose noticeably stimulated growth yields of L. acidophilus NCFM, and additionally, β-d-glucosyl-(1,4)-xylose stimulated B. longum Bl-05. α-Glucosyl-(1,4)-glucosamine and α-glucosyl-(1,4)-N-acetyl-glucosamine enhanced the growth rate of B. animalis subsp. lactis and B. longum Bl-05, whereas L. acidophilus NCFM and Bac. vulgatus did not grow on these OS. α-Galactosyl-(1,6)-α-galactosyl-(1,6)-glucose advanced the growth rate of B. animalis subsp. lactis and L. acidophilus NCFM. Thus several of the structurally well-defined OS supported growth of beneficial gut bacteria. This reflects a broad specificity of their sugar transporters for OS, including specificity for non-naturally occurring OS, hence showing promise for design of novel prebiotics.

U2 - 10.1039/C3FO30357H

DO - 10.1039/C3FO30357H

M3 - Journal article

VL - 4

SP - 784

EP - 793

JO - Food & Function

JF - Food & Function

SN - 2042-6496

IS - 5

ER -