In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets

Marta S. Silva, P Antony Jesu Prabhu, Robin Ørnsrud*, Veronika Sele, Saskia Kröckel, Jens Jørgen Sloth, Heidi Amlund

*Corresponding author for this work

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Abstract

The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species. This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite and manganous sulphate and an organic mineral (OM) diet was supplemented with zinc chelate of glycine, l-selenomethionine and manganese chelate of glycine. The solubility of Zn was similar in both diets tested. The amount of soluble Zn was low in the acidic hydrolysis (3-8%) and lower in the alkaline hydrolysis (0.4-2%). The solubility of Se was higher in the OM diet (7-34%) compared with the IM diet (3-12%). Regarding Mn, after the acidic hydrolysis the solubility was higher in the IM diet (6-25%) than the OM diet (4-17%). The in vitro solubility were compared with in vivo availability of Zn, Se and Mn. Data obtained for solubility (%) of Zn, Se and Mn was lower when compared with apparent availability (%) of Zn, Se and Mn. Data obtained demonstrated that solubility of Zn, Se and Mn was influenced by the mineral chemical form supplemented to the diet and by the gastrointestinal environment. The solubility of Zn, Se and Mn was not comparable with the apparent availability of Zn, Se and Mn. Nevertheless, the effect of the chemical form of the minerals was similar for the solubility of Zn, Se and Mn and the apparent availability of Zn, Se and Mn. Considering the overall results of this study, the in vitro method could replace some of the in vivo studies for a qualitative evaluation but not for a quantitative evaluation.
Original languageEnglish
Article number126418
JournalJournal of Trace Elements in Medicine and Biology
Volume57
Number of pages8
ISSN0946-672X
DOIs
Publication statusPublished - 2020

Keywords

  • Availability
  • Hydrolysis
  • Minerals
  • In vitro method
  • Accessibility
  • Fish nutrition

Cite this

@article{144d05dfc3814273913c326a2b71a099,
title = "In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets",
abstract = "The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species. This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite and manganous sulphate and an organic mineral (OM) diet was supplemented with zinc chelate of glycine, l-selenomethionine and manganese chelate of glycine. The solubility of Zn was similar in both diets tested. The amount of soluble Zn was low in the acidic hydrolysis (3-8{\%}) and lower in the alkaline hydrolysis (0.4-2{\%}). The solubility of Se was higher in the OM diet (7-34{\%}) compared with the IM diet (3-12{\%}). Regarding Mn, after the acidic hydrolysis the solubility was higher in the IM diet (6-25{\%}) than the OM diet (4-17{\%}). The in vitro solubility were compared with in vivo availability of Zn, Se and Mn. Data obtained for solubility ({\%}) of Zn, Se and Mn was lower when compared with apparent availability ({\%}) of Zn, Se and Mn. Data obtained demonstrated that solubility of Zn, Se and Mn was influenced by the mineral chemical form supplemented to the diet and by the gastrointestinal environment. The solubility of Zn, Se and Mn was not comparable with the apparent availability of Zn, Se and Mn. Nevertheless, the effect of the chemical form of the minerals was similar for the solubility of Zn, Se and Mn and the apparent availability of Zn, Se and Mn. Considering the overall results of this study, the in vitro method could replace some of the in vivo studies for a qualitative evaluation but not for a quantitative evaluation.",
keywords = "Availability, Hydrolysis, Minerals, In vitro method, Accessibility, Fish nutrition",
author = "Silva, {Marta S.} and Prabhu, {P Antony Jesu} and Robin {\O}rnsrud and Veronika Sele and Saskia Kr{\"o}ckel and Sloth, {Jens J{\o}rgen} and Heidi Amlund",
year = "2020",
doi = "10.1016/j.jtemb.2019.126418",
language = "English",
volume = "57",
journal = "Journal of Trace Elements in Medicine and Biology",
issn = "0946-672X",
publisher = "Elsevier",

}

In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets. / Silva, Marta S.; Prabhu, P Antony Jesu; Ørnsrud, Robin; Sele, Veronika; Kröckel, Saskia; Sloth, Jens Jørgen; Amlund, Heidi.

In: Journal of Trace Elements in Medicine and Biology, Vol. 57, 126418, 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets

AU - Silva, Marta S.

AU - Prabhu, P Antony Jesu

AU - Ørnsrud, Robin

AU - Sele, Veronika

AU - Kröckel, Saskia

AU - Sloth, Jens Jørgen

AU - Amlund, Heidi

PY - 2020

Y1 - 2020

N2 - The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species. This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite and manganous sulphate and an organic mineral (OM) diet was supplemented with zinc chelate of glycine, l-selenomethionine and manganese chelate of glycine. The solubility of Zn was similar in both diets tested. The amount of soluble Zn was low in the acidic hydrolysis (3-8%) and lower in the alkaline hydrolysis (0.4-2%). The solubility of Se was higher in the OM diet (7-34%) compared with the IM diet (3-12%). Regarding Mn, after the acidic hydrolysis the solubility was higher in the IM diet (6-25%) than the OM diet (4-17%). The in vitro solubility were compared with in vivo availability of Zn, Se and Mn. Data obtained for solubility (%) of Zn, Se and Mn was lower when compared with apparent availability (%) of Zn, Se and Mn. Data obtained demonstrated that solubility of Zn, Se and Mn was influenced by the mineral chemical form supplemented to the diet and by the gastrointestinal environment. The solubility of Zn, Se and Mn was not comparable with the apparent availability of Zn, Se and Mn. Nevertheless, the effect of the chemical form of the minerals was similar for the solubility of Zn, Se and Mn and the apparent availability of Zn, Se and Mn. Considering the overall results of this study, the in vitro method could replace some of the in vivo studies for a qualitative evaluation but not for a quantitative evaluation.

AB - The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species. This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite and manganous sulphate and an organic mineral (OM) diet was supplemented with zinc chelate of glycine, l-selenomethionine and manganese chelate of glycine. The solubility of Zn was similar in both diets tested. The amount of soluble Zn was low in the acidic hydrolysis (3-8%) and lower in the alkaline hydrolysis (0.4-2%). The solubility of Se was higher in the OM diet (7-34%) compared with the IM diet (3-12%). Regarding Mn, after the acidic hydrolysis the solubility was higher in the IM diet (6-25%) than the OM diet (4-17%). The in vitro solubility were compared with in vivo availability of Zn, Se and Mn. Data obtained for solubility (%) of Zn, Se and Mn was lower when compared with apparent availability (%) of Zn, Se and Mn. Data obtained demonstrated that solubility of Zn, Se and Mn was influenced by the mineral chemical form supplemented to the diet and by the gastrointestinal environment. The solubility of Zn, Se and Mn was not comparable with the apparent availability of Zn, Se and Mn. Nevertheless, the effect of the chemical form of the minerals was similar for the solubility of Zn, Se and Mn and the apparent availability of Zn, Se and Mn. Considering the overall results of this study, the in vitro method could replace some of the in vivo studies for a qualitative evaluation but not for a quantitative evaluation.

KW - Availability

KW - Hydrolysis

KW - Minerals

KW - In vitro method

KW - Accessibility

KW - Fish nutrition

U2 - 10.1016/j.jtemb.2019.126418

DO - 10.1016/j.jtemb.2019.126418

M3 - Journal article

C2 - 31704176

VL - 57

JO - Journal of Trace Elements in Medicine and Biology

JF - Journal of Trace Elements in Medicine and Biology

SN - 0946-672X

M1 - 126418

ER -