In utero exposure to nanosized carbon black (Printex90) does not induce tandem repeat mutations in female murine germ cells

Anne Mette Zenner Boisen, Thomas Shipley, Petra Jackson, Håkan Wallin, Christine Lydia Nellemann, Ulla Vogel, Carole L. Yauk, Karin Sørig Hougaard

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Inhalation of particles has been shown to induce mutations in the male germline in mice following both prenatal and adult exposures in several experiments. In contrast, the effects of particles on female germ cell mutagenesis are not well established. Germline mutations are induced during active cell division, which occurs during fetal development in females. We investigated the effects of prenatal exposure to carbon black nanoparticles (CB) on induction of mutations in the female mouse germline during fetal development, spanning the critical developmental stages of oogenesis. Pregnant C57BL/6J mice were exposed four times during gestation by intratracheal instillation of 67μg/animal of nanosized carbon black Printex90 or vehicle (gestation days 7, 10, 15 and 18). Female offspring were raised to maturity and mated with unexposed CBA males. Expanded simple tandem repeat (ESTR) germline mutation rates in the resulting F2 generation were determined from full pedigrees (mother, father, offspring) of F1 female mice (178 CB-exposed and 258 control F2 offspring). ESTR mutation rates in CB-exposed F2 female offspring were not statistically different from those of F2 female control offspring.
Original languageEnglish
JournalReproductive Toxicology
Issue numberSI
Pages (from-to)45-48
Publication statusPublished - 2013
Event41st European Teratology Society Conference - Stresa, Italy
Duration: 8 Sept 201311 Sept 2013
Conference number: 41


Conference41st European Teratology Society Conference


  • CB - carbon black Printex90
  • ESTR - expanded simple tandem repeat
  • F1 - first generation
  • F2 - second generation
  • GD - gestation day
  • P - parental generation
  • PND - postnatal day
  • ROS - reactive oxygen species


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