In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D)

Xueling Quan, Arto Heiskanen, Maria Tenje, Anja Boisen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (Epzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results, we present a model to explain the influence of the potential pulsing. Aside fromthe effect of applied potentials on DNA related processes, this work also demonstrates the versatility of the combination of electrochemistry and quartz crystal microbalance with dissipation in facilitating real-time in situ monitoring of such processes.
    Original languageEnglish
    JournalElectrochemistry Communications
    Volume48
    Pages (from-to)111-114
    Number of pages4
    ISSN1388-2481
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Potential pulse assisted functionalization
    • Thiolated DNA functionalization
    • DNA hybridization
    • Vertically aligned DNA conformation
    • EQCM-D

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