Abstract
The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with
dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (Epzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential
to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results,
we present a model to explain the influence of the potential pulsing. Aside fromthe effect of applied potentials on DNA related processes, this work also demonstrates the versatility of the combination of electrochemistry and
quartz crystal microbalance with dissipation in facilitating real-time in situ monitoring of such processes.
Original language | English |
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Journal | Electrochemistry Communications |
Volume | 48 |
Pages (from-to) | 111-114 |
Number of pages | 4 |
ISSN | 1388-2481 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- Potential pulse assisted functionalization
- Thiolated DNA functionalization
- DNA hybridization
- Vertically aligned DNA conformation
- EQCM-D