In situ examination of cell growth and death of Lactococcus lactis

Mia Ryssel, Zhi Duan, Henrik Siegumfeldt

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

This study enables in situ studying of the growth and death of a large number of individual cells in a solid matrix. A wild type of Lactococcus lactis and several mutants with varying expression of GuaB was investigated. Large variability in the final size of individual microcolonies arising from clonal cells was observed. However, when growth was averaged over 16 locations in a specimen, the SEM was small and notable differences could be observed between the investigated strains, where mutants with lower expression of GuaB had a slower growth rate. The results show that the slow-growing mutants exhibited a lower fraction of dead cells, which indicate that slow-growing mutants are slightly more robust than the faster-growing strains. The large variability in the final size of individual microcolonies arising from clonal cells was quite surprising. We suggest that the control of the size of a microcolony is, at least partially, related to the actual microcolony depended on phenotypic heterogeneity. These findings are important to consider whenever a solid medium with discrete microcolonies is investigated.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume343
Issue number1
Pages (from-to)82
ISSN0378-1097
DOIs
Publication statusPublished - 2013
Externally publishedYes

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