In-situ biogas upgrading assisted by bioaugmentation with hydrogenotrophic methanogens during mesophilic and thermophilic co-digestion

Matteo Palù, Maria Peprah, Panagiotis Tsapekos, Panagiotis Kougias, Stefano Campanaro*, Irini Angelidaki, Laura Treu

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In this study, the effects of bioaugmentation of typically dominant hydrogenotrophic methanogens to CSTR co-digesting cheese whey and manure, under in-situ biomethanation operations were investigated. Reactors working at mesophilic (37°C) and thermophilic (55°C) conditions were independently treated and examined in terms of microbial composition and process dynamics. Addition of Methanoculleus bourgensis in the mesophilic reactor led to a stable biomethanation, and an improved microbial metabolism, resulting in 11% increase in CH4 production rate. 16S rRNA and biochemical analyses revealed an enrichment in syntrophic and acidogenic species abundance. Moreover, nearly total volatile fatty acids conversion was observed. Differently, Methanothermobacter thermautotrophicus addition in the thermophilic reactor did not promote biogas upgrading performance due to incomplete H2 conversion and inefficient community adaptation to H2 excess, ultimately favoring acetoclastic methanogenesis. Bioaugmentation constitutes a viable tool to strengthen in-situ upgrading processes and paves the way to the development of more sophisticated and robust microbial inoculants.
Original languageEnglish
Article number126754
JournalBioresource Technology
Volume348
Number of pages13
ISSN0960-8524
DOIs
Publication statusPublished - 2022

Keywords

  • Anaerobic digestion
  • Bioaugmentation
  • Biogas upgrading
  • Hydrogenotrophic methanogens
  • Microbiome

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