In situ atomic force microscope imaging of supported lipid bilayers

Thomas Kaasgaard; Chad Leidy; John Hjorth Ipsen; Ole G. Mouritsen; Kent Jørgensen

In situ atomic force microscope imaging of supported lipid bilayers

Thomas Kaasgaard, Chad Leidy, John Hjorth Ipsen, Ole G. Mouritsen, Kent Jørgensen

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In situ AFM images of phospholipase A/sub 2/ (PLA/sub 2/) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA/sub 2/-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P/sub beta /-ripple phase is observed.

Original language	English
Journal	Single Molecules
Volume	2
Issue number	2
Pages (from-to)	105-108
ISSN	1438-5163
Publication status	Published - 2001

OpenUrl availability

Full text

Cite this

@article{55fd189f41ac483d9af5b3c22ecbfe37,

title = "In situ atomic force microscope imaging of supported lipid bilayers",

abstract = "In situ AFM images of phospholipase A/sub 2/ (PLA/sub 2/) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA/sub 2/-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P/sub beta /-ripple phase is observed.",

author = "Thomas Kaasgaard and Chad Leidy and Ipsen, {John Hjorth} and Mouritsen, {Ole G.} and Kent J{\o}rgensen",

year = "2001",

language = "English",

volume = "2",

pages = "105--108",

journal = "Single Molecules",

issn = "1438-5163",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

number = "2",

}

TY - JOUR

T1 - In situ atomic force microscope imaging of supported lipid bilayers

AU - Kaasgaard, Thomas

AU - Leidy, Chad

AU - Ipsen, John Hjorth

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2001

Y1 - 2001

N2 - In situ AFM images of phospholipase A/sub 2/ (PLA/sub 2/) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA/sub 2/-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P/sub beta /-ripple phase is observed.

AB - In situ AFM images of phospholipase A/sub 2/ (PLA/sub 2/) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA/sub 2/-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P/sub beta /-ripple phase is observed.

M3 - Journal article

SN - 1438-5163

VL - 2

SP - 105

EP - 108

JO - Single Molecules

JF - Single Molecules

IS - 2

ER -

In situ atomic force microscope imaging of supported lipid bilayers

Abstract

OpenUrl availability

Fingerprint

Cite this