In situ atomic force microscope imaging of supported lipid bilayers

Thomas Kaasgaard, Chad Leidy, John Hjorth Ipsen, Ole G. Mouritsen, Kent Jørgensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In situ AFM images of phospholipase A/sub 2/ (PLA/sub 2/) hydrolysis of mica-supported one- and two-component lipid bilayers are presented. For one-component DPPC bilayers an enhanced enzymatic activity is observed towards preexisting defects in the bilayer. Phase separation is observed in two-component DMPC-DSPC bilayers and a remarkable enhanced hydrolytic activity of the PLA/sub 2/-enzyme for the DMPC-rich phase is seen. Furthermore, in a supported double bilayer system a characteristic ripple structure, most likely related to the formation of the P/sub beta /-ripple phase is observed.
Original languageEnglish
JournalSingle Molecules
Volume2
Issue number2
Pages (from-to)105-108
ISSN1438-5163
Publication statusPublished - 2001

Cite this

Kaasgaard, T., Leidy, C., Ipsen, J. H., Mouritsen, O. G., & Jørgensen, K. (2001). In situ atomic force microscope imaging of supported lipid bilayers. Single Molecules, 2(2), 105-108.