In-house characterization of protein powder

Christian Grundahl Hartmann, Pernille Harris, Kenny Ståhl

Research output: Contribution to journalConference articleResearchpeer-review

Abstract

Collecting protein powder diffraction data on standard in-house powder diffractometers requires careful handling of the samples. Specially designed sample holders combined with optimized collimation were found to be the key factors in improving the data quality and reducing the data collection time. For safe identification of the crystal form the experimental patterns have to be compared with patterns calculated from known crystal structures. Very good agreement with Protein Data Bank data was obtained after including corrections for background, unit cell parameters, disordered bulk-solvent, and geometric factors. The data collection and correction procedures were demonstrated by the identification of three different crystal forms of insulin.
Original languageEnglish
JournalZeitschrift für Kristallographie Proceedings
Volume1-2
Issue number1
Pages (from-to)163-168
ISSN1869-1315
Publication statusPublished - 2011
Event12th European Powder Diffraction Conference - Darmstadt, Germany
Duration: 27 Aug 201030 Aug 2010
Conference number: 12
http://www.conventus.de/epdic12/

Conference

Conference12th European Powder Diffraction Conference
Number12
CountryGermany
CityDarmstadt
Period27/08/201030/08/2010
Internet address

Keywords

  • In-house powder diffraction
  • Bulk-solvent correction
  • Protein

Cite this

Hartmann, C. G., Harris, P., & Ståhl, K. (2011). In-house characterization of protein powder. Zeitschrift für Kristallographie Proceedings, 1-2(1), 163-168.