Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate

Shun Zhi Wang, Zi Kai Wang, Jin Song Gong*, Jiufu Qin, Ting Ting Dong, Zheng Hong Xu, Jin Song Shi

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance. The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3. The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively. The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively. The storage stability of the composite immobilized cells was slightly increased. The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L−1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L−1 nicotinic acid.
Original languageEnglish
JournalBioprocess and Biosystems Engineering
Volume43
Issue number12
Pages (from-to)2201-2207
Number of pages7
ISSN1615-7591
DOIs
Publication statusPublished - 2020

Keywords

  • Biocatalysis
  • Co-immobilization
  • Nitrilase
  • Inorganic materials

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