Improvement of trans-sialylation versus hydrolysis activity of an engineered sialidase from Trypanosoma rangeli by use of co-solvents.

Birgitte Zeuner, Anders Riisager, Jørn Dalgaard Mikkelsen, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Biocatalytic trans-sialylation is relevant for the design of biomimetic oligosaccharides such as human milk oligosaccharides. t-Butanol and ionic liquids, EAN (ethylammonium nitrate), [MMIm][MeSO4] (1,3-dimethylimidazolium methyl sulfate), and [C2OHMIm][PF6] (1-(2-hydroxyethyl)-3-methylimidazolium hexafluorophosphate), were examined as co-solvents for the improvement of the synthesis versus hydrolysis ratio in the trans-sialylation of lactose, catalysed by an engineered sialidase from Trypanosoma rangeli. The use of 25 % (v/v) t-butanol as co-solvent significantly increased 3'-sialyllactose production by 40 % from 1.04 ± 0.09 to 1.47 ± 0.01 mM. The synthesis versus hydrolysis ratio increased correspondingly by 1.2-times. 1-2.5 % (v/v) EAN or [C2OHMIm][PF6] improved the synthesis versus hydrolysis ratio up to 2.5-times but simultaneously decreased the 3'-sialyllactose yield, probably due to enzyme inactivation caused by the ionic liquid. [MMIm][MeSO4] had a detrimental effect on the trans-sialylation yield and on the ratio between synthesis and hydrolysis.
Original languageEnglish
JournalBiotechnology Letters
Volume36
Issue number6
Pages (from-to)1315-1320
Number of pages6
ISSN0141-5492
DOIs
Publication statusPublished - 2014

Keywords

  • HASH(0x4772848)
  • t-Butanol
  • Casein glycomacropeptide (CGMP)
  • Co-solvent
  • Ionic liquids
  • 3′-Sialyllactose
  • trans-Sialidase
  • Trypanosoma rangeli

Fingerprint Dive into the research topics of 'Improvement of trans-sialylation versus hydrolysis activity of an engineered sialidase from Trypanosoma rangeli by use of co-solvents.'. Together they form a unique fingerprint.

Cite this