Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis

M. Ohlin, A. Fornell, Henrik Bruus, M. Tenje

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, φ = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.
Original languageEnglish
Article number084002
JournalJournal of Micromechanics and Microengineering
Volume27
Issue number8
Number of pages8
ISSN0960-1317
DOIs
Publication statusPublished - 2017

Keywords

  • Acoustophoresis
  • Droplet microfluids
  • Unltrasonic standing wave
  • Microparticle detection
  • Microparticle manipulation

Cite this

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title = "Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis",
abstract = "We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, φ = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4){\%} of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1){\%} for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.",
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author = "M. Ohlin and A. Fornell and Henrik Bruus and M. Tenje",
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doi = "10.1088/1361-6439/aa7967",
language = "English",
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journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",
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Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis. / Ohlin, M.; Fornell, A.; Bruus, Henrik; Tenje, M.

In: Journal of Micromechanics and Microengineering, Vol. 27, No. 8, 084002, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis

AU - Ohlin, M.

AU - Fornell, A.

AU - Bruus, Henrik

AU - Tenje, M.

PY - 2017

Y1 - 2017

N2 - We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, φ = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

AB - We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, φ = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

KW - Acoustophoresis

KW - Droplet microfluids

KW - Unltrasonic standing wave

KW - Microparticle detection

KW - Microparticle manipulation

U2 - 10.1088/1361-6439/aa7967

DO - 10.1088/1361-6439/aa7967

M3 - Journal article

VL - 27

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 8

M1 - 084002

ER -