Improved coupling of bacterial polysaccharides to macromolecules and solid supports

Ulrik Boas (Inventor)

    Research output: Patent

    60 Downloads (Pure)

    Abstract

    The invention relates to a method of producing a polysaccharide-carrier conjugate comprising coupling a polysaccharide to a carrier, said polysaccharide comprising at least one monosaccharide unit comprising a keto-carboxy group according to the formula -C(=O)COOR, where R is either hydrogen or C1-C6 alkyl or a ringclosed ketal or hemi-ketal corresponding thereto, said method comprising the steps of, i) providing a carrier comprising N-hydroxylamine- or N-alkoxyamine groups according to the formula -NHOR, wherein R is H or C1-C6 alkyl attached thereto, ii) reacting said N-hydroxylamine or N-alkoxyamine group of the carrier with a keto-carboxy group of said polysaccharide to form a covalent amide bond between the carrier and the polysaccharide. Another aspect of the present invention relates to a compound or solid surface obtained when performing the method of the present invention. A third aspect of the present invention relates to the use of the compound or solid surface in the preparation of an assay device for the detection of antibodies against gram negative bacteria.
    Original languageEnglish
    IPCG01N33/569
    Patent numberWO2013060335
    Filing date24/10/2012
    CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
    Publication statusPublished - 2013

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