Described is a new biosensor-based detection system for effector compounds, useful for in vivo applications in e.g. screening and selecting of cells which produce a small molecule effector compound or which take up a small molecule effector compound from its environment. The detection system comprises a protein or RNA-based biosensor for the effector compound which indirectly regulates the expression of a reporter gene via two hybrid proteins, providing for fewer false signals or less 'noise', tuning of sensitivity or other advantages over conventional systems where the biosensor directly regulates the reporter gene.
|IPC||C12Q 1/ 68 A I|
|Country||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 2 Apr 2015|