Implications of high-temperature events and water deficits on protein profiles in wheat (Triticum aestivum L. cv. Vinjett) grain

Fen Yang, Anders Dysted Jørgensen, Huawei Li, Ib Søndergaard, Christine Finnie, Birte Svensson, Dong Jiang, Bernd Wollenweber, Susanne Jacobsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are reported of interaction of water deficits and/or a high-temperature event (32 degrees C) during vegetative growth (terminal spikelet) with either of these stress events applied during generative growth (anthesis) in wheat. Influence of combinations of stress on protein fractions (albumins, globulins, gliadins and glutenins) in grains and stress-induced changes on the albumin and gliadin proteomes were investigated by 2-DE and MS. The synthesis of individual protein fractions was shown to be affected by both the type and time of the applied stresses. Identified drought or high-temperature-responsive proteins included proteins involved in primary metabolism, storage and stress response such as late embryogenesis abundant proteins, peroxiredoxins and alpha-amylase/trypsin inhibitors. Several proteins, e.g. heat shock protein and 14-3-3 protein changed in abundance only under multiple high temperatures.
    Original languageEnglish
    JournalProteomics
    Volume11
    Issue number9, Sp. Iss. SI
    Pages (from-to)1684-1695
    ISSN1615-9853
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Plant proteomics
    • Grain protein fractions
    • Environmental stress
    • Wheat

    Cite this

    Yang, F., Jørgensen, A. D., Li, H., Søndergaard, I., Finnie, C., Svensson, B., Jiang, D., Wollenweber, B., & Jacobsen, S. (2011). Implications of high-temperature events and water deficits on protein profiles in wheat (Triticum aestivum L. cv. Vinjett) grain. Proteomics, 11(9, Sp. Iss. SI), 1684-1695. https://doi.org/10.1002/pmic.201000654