Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisiae

Verena Siewers, Rita San-Bento, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Saccharomyces cerevisiae has in several cases been proven to be a suitable host for the production of natural products and was recently exploited for the production of non-ribosomal peptides. Synthesis of non-ribosomal peptides (NRPs) is mediated by NRP synthetases (NRPSs), modular enzymes, which are often organized in enzyme complexes. In these complexes, partner NRPSs interact via communication-mediating domains (COM domains). In order to test whether functional interaction between separate NRPS modules is possible in yeast we constructed a yeast strain expressing two modules with compatible COM domains from two plasmids. Successful production as well as secretion of the expected dipeptide was detected. This opens the possibility of using yeast as a eukaryotic platform for fast assessment of new module combinations for the development of novel NRP compounds.
    Original languageEnglish
    JournalBiotechnology and Bioengineering (Print)
    Volume106
    Issue number5
    Pages (from-to)841-844
    ISSN0006-3592
    DOIs
    Publication statusPublished - 2010

    Keywords

    • secondary metabolism
    • non-ribosomal peptide synthetase
    • yeast
    • COM domain

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