Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks

Chiara Canali; Soumyaranjan Mohanty; Arto Heiskanen; Haseena Bashir Muhammad; Ørjan Grøttem Martinsen; Martin Dufva; Anders Wolff; Jenny Emnéus

doi:10.1002/elan.201400413

Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks

Chiara Canali, Soumyaranjan Mohanty, Arto Heiskanen, Haseena Bashir Muhammad, Ørjan Grøttem Martinsen, Martin Dufva, Anders Wolff, Jenny Emnéus

University of Oslo

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.

Original language	English
Journal	Electroanalysis
Volume	27
Pages (from-to)	193-199
ISSN	1040-0397
DOIs	https://doi.org/10.1002/elan.201400413
Publication status	Published - 2015

Keywords

Impedance spectroscopy
3D cell culture scaffold
Porosity
Channel networks
3D printing

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title = "Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks",

abstract = "We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.",

keywords = "Impedance spectroscopy, 3D cell culture scaffold, Porosity, Channel networks, 3D printing",

author = "Chiara Canali and Soumyaranjan Mohanty and Arto Heiskanen and Muhammad, {Haseena Bashir} and Martinsen, {{\O}rjan Gr{\o}ttem} and Martin Dufva and Anders Wolff and Jenny Emn{\'e}us",

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doi = "10.1002/elan.201400413",

language = "English",

volume = "27",

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T1 - Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks

AU - Canali, Chiara

AU - Mohanty, Soumyaranjan

AU - Heiskanen, Arto

AU - Muhammad, Haseena Bashir

AU - Martinsen, Ørjan Grøttem

AU - Dufva, Martin

AU - Wolff, Anders

AU - Emnéus, Jenny

PY - 2015

Y1 - 2015

N2 - We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.

AB - We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.

KW - Impedance spectroscopy

KW - 3D cell culture scaffold

KW - Porosity

KW - Channel networks

KW - 3D printing

U2 - 10.1002/elan.201400413

DO - 10.1002/elan.201400413

M3 - Journal article

SN - 1040-0397

VL - 27

SP - 193

EP - 199

JO - Electroanalysis

JF - Electroanalysis

ER -

Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks

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Keywords

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