Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks

Chiara Canali, Soumyaranjan Mohanty, Arto Heiskanen, Haseena Bashir Muhammad, Ørjan Grøttem Martinsen, Martin Dufva, Anders Wolff, Jenny Emnéus

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.
    Original languageEnglish
    JournalElectroanalysis
    Volume27
    Pages (from-to)193-199
    ISSN1040-0397
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Impedance spectroscopy
    • 3D cell culture scaffold
    • Porosity
    • Channel networks
    • 3D printing

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