Impedance flow cytometry for viability analysis of Corynebacterium glutamicum

Fabian Stefan Franz Hartmann, Ioannis Anastasiou, Tamara Weiß, Jing Shen, Gerd Michael Seibold*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Corynebacterium glutamicum efficiently produces glutamate when growth is inhibited. Analyses of viability in this non-growing state requires time consuming plating and determination of colony forming units. We here establish impedance flow cytometry measurements to assess the viability of non-growing, glutamate producing C. glutamicum cultures within minutes.
Original languageEnglish
Article number106347
JournalJournal of Microbiological methods
Volume191
Number of pages4
ISSN0167-7012
DOIs
Publication statusPublished - 2021

Keywords

  • Cell wall inhibitor
  • Corynebacterium glutamicum
  • Glutamate production
  • Impedance flow cytometry
  • Penicillin
  • Viability

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