Impact of Rap-Phr system abundance on adaptation of Bacillus subtilis

Ramses Gallegos-Monterrosa, Mathilde Nordgaard Christensen, Tino Barchewitz, Sonja Koppenhöfer, B. Priyadarshini, Balázs Bálint, Gergely Maróti, Paul J. Kempen, Anna Dragoš, Ákos T. Kovács*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Microbes commonly display great genetic plasticity, which has allowed them to colonize all ecological niches on Earth. Bacillus subtilis is a soil-dwelling organism that can be isolated from a wide variety of environments. An interesting characteristic of this bacterium is its ability to form biofilms that display complex heterogeneity: individual, clonal cells develop diverse phenotypes in response to different environmental conditions within the biofilm. Here, we scrutinized the impact that the number and variety of the Rap-Phr family of regulators and cell-cell communication modules of B. subtilis has on genetic adaptation and evolution. We examine how the Rap family of phosphatase regulators impacts sporulation in diverse niches using a library of single and double rap-phr mutants in competition under 4 distinct growth conditions. Using specific DNA barcodes and whole-genome sequencing, population dynamics were followed, revealing the impact of individual Rap phosphatases and arising mutations on the adaptability of B. subtilis.
Original languageEnglish
Article number468
JournalCommunications Biology
Volume4
Issue number1
Number of pages12
ISSN2399-3642
DOIs
Publication statusPublished - 2021

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