Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure

Xiaofang Pan, Lina Wang, Nan Lv, Jing Ning, Mingdian Zhou, Tao Wang, Chunxing Li, Gefu Zhu*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis. Thus, to evaluate the effect of granular physical structure on methanogenesis and methanogenic community variation, intact and disintegrated granules were applied as inocula with formate, hydrogen and acetate as sole substrates in batch reactors. Kinetics results revealed that the physical structure of sludge had little impact on methane yield potential from three substrates, while a significantly different impact on methanogenesis rates of formate, hydrogen and acetate. The methanogenesis rate of formate in disintegrated granules was higher than that in the intact granular system, the methanogenesis rate of H2/CO2 in the intact granular system was higher than that in the disintegrated granules and the methanogenesis rate of acetate was similar with the in intact and disintegrated granular systems. Besides, in both intact and disintegrated granular systems, methanogenesis rates of formate were the highest, then followed the H2/CO2 and acetate was the lowest, indicating formate consumption has an advantage over hydrogen in the studied system. A microbial assay indicated that Methanobacteriales, Methanosarcinales and Methanomicrobiales are dominant methanogens on the order level, and the physical structure of granular sludge has little influence on methanogenic communities on the order level but showed significant influence on the species level. It enlightens us that the physical structure of sludge could be considered for regulating the anaerobic digestion via influencing the methanogenesis rates.

Original languageEnglish
JournalRSC Advances
Volume9
Issue number51
Pages (from-to)29570-29578
ISSN2046-2069
DOIs
Publication statusPublished - 2019

Cite this

Pan, X., Wang, L., Lv, N., Ning, J., Zhou, M., Wang, T., ... Zhu, G. (2019). Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure. RSC Advances, 9(51), 29570-29578. https://doi.org/10.1039/c9ra04257a
Pan, Xiaofang ; Wang, Lina ; Lv, Nan ; Ning, Jing ; Zhou, Mingdian ; Wang, Tao ; Li, Chunxing ; Zhu, Gefu. / Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure. In: RSC Advances. 2019 ; Vol. 9, No. 51. pp. 29570-29578.
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title = "Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure",
abstract = "Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis. Thus, to evaluate the effect of granular physical structure on methanogenesis and methanogenic community variation, intact and disintegrated granules were applied as inocula with formate, hydrogen and acetate as sole substrates in batch reactors. Kinetics results revealed that the physical structure of sludge had little impact on methane yield potential from three substrates, while a significantly different impact on methanogenesis rates of formate, hydrogen and acetate. The methanogenesis rate of formate in disintegrated granules was higher than that in the intact granular system, the methanogenesis rate of H2/CO2 in the intact granular system was higher than that in the disintegrated granules and the methanogenesis rate of acetate was similar with the in intact and disintegrated granular systems. Besides, in both intact and disintegrated granular systems, methanogenesis rates of formate were the highest, then followed the H2/CO2 and acetate was the lowest, indicating formate consumption has an advantage over hydrogen in the studied system. A microbial assay indicated that Methanobacteriales, Methanosarcinales and Methanomicrobiales are dominant methanogens on the order level, and the physical structure of granular sludge has little influence on methanogenic communities on the order level but showed significant influence on the species level. It enlightens us that the physical structure of sludge could be considered for regulating the anaerobic digestion via influencing the methanogenesis rates.",
author = "Xiaofang Pan and Lina Wang and Nan Lv and Jing Ning and Mingdian Zhou and Tao Wang and Chunxing Li and Gefu Zhu",
year = "2019",
doi = "10.1039/c9ra04257a",
language = "English",
volume = "9",
pages = "29570--29578",
journal = "R S C Advances",
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Pan, X, Wang, L, Lv, N, Ning, J, Zhou, M, Wang, T, Li, C & Zhu, G 2019, 'Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure', RSC Advances, vol. 9, no. 51, pp. 29570-29578. https://doi.org/10.1039/c9ra04257a

Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure. / Pan, Xiaofang; Wang, Lina; Lv, Nan; Ning, Jing; Zhou, Mingdian; Wang, Tao; Li, Chunxing; Zhu, Gefu.

In: RSC Advances, Vol. 9, No. 51, 2019, p. 29570-29578.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Impact of physical structure of granular sludge on methanogenesis and methanogenic community structure

AU - Pan, Xiaofang

AU - Wang, Lina

AU - Lv, Nan

AU - Ning, Jing

AU - Zhou, Mingdian

AU - Wang, Tao

AU - Li, Chunxing

AU - Zhu, Gefu

PY - 2019

Y1 - 2019

N2 - Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis. Thus, to evaluate the effect of granular physical structure on methanogenesis and methanogenic community variation, intact and disintegrated granules were applied as inocula with formate, hydrogen and acetate as sole substrates in batch reactors. Kinetics results revealed that the physical structure of sludge had little impact on methane yield potential from three substrates, while a significantly different impact on methanogenesis rates of formate, hydrogen and acetate. The methanogenesis rate of formate in disintegrated granules was higher than that in the intact granular system, the methanogenesis rate of H2/CO2 in the intact granular system was higher than that in the disintegrated granules and the methanogenesis rate of acetate was similar with the in intact and disintegrated granular systems. Besides, in both intact and disintegrated granular systems, methanogenesis rates of formate were the highest, then followed the H2/CO2 and acetate was the lowest, indicating formate consumption has an advantage over hydrogen in the studied system. A microbial assay indicated that Methanobacteriales, Methanosarcinales and Methanomicrobiales are dominant methanogens on the order level, and the physical structure of granular sludge has little influence on methanogenic communities on the order level but showed significant influence on the species level. It enlightens us that the physical structure of sludge could be considered for regulating the anaerobic digestion via influencing the methanogenesis rates.

AB - Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis. Thus, to evaluate the effect of granular physical structure on methanogenesis and methanogenic community variation, intact and disintegrated granules were applied as inocula with formate, hydrogen and acetate as sole substrates in batch reactors. Kinetics results revealed that the physical structure of sludge had little impact on methane yield potential from three substrates, while a significantly different impact on methanogenesis rates of formate, hydrogen and acetate. The methanogenesis rate of formate in disintegrated granules was higher than that in the intact granular system, the methanogenesis rate of H2/CO2 in the intact granular system was higher than that in the disintegrated granules and the methanogenesis rate of acetate was similar with the in intact and disintegrated granular systems. Besides, in both intact and disintegrated granular systems, methanogenesis rates of formate were the highest, then followed the H2/CO2 and acetate was the lowest, indicating formate consumption has an advantage over hydrogen in the studied system. A microbial assay indicated that Methanobacteriales, Methanosarcinales and Methanomicrobiales are dominant methanogens on the order level, and the physical structure of granular sludge has little influence on methanogenic communities on the order level but showed significant influence on the species level. It enlightens us that the physical structure of sludge could be considered for regulating the anaerobic digestion via influencing the methanogenesis rates.

U2 - 10.1039/c9ra04257a

DO - 10.1039/c9ra04257a

M3 - Journal article

VL - 9

SP - 29570

EP - 29578

JO - R S C Advances

JF - R S C Advances

SN - 2046-2069

IS - 51

ER -