TY - JOUR
T1 - Immunoproteasomes are essential for clearance of Listeria monocytogenes in nonlymphoid tissues but not for induction of bacteria-specific CD8(+) T cells
AU - Strehl, Britta
AU - Joeris, Thorsten
AU - Rieger, Melanie
AU - Visekruna, Alexander
AU - Textoris-Taube, Kathrin
AU - Kaufmann, Stefan H. E.
AU - Kloetzel, Peter-Michael
AU - Kuckelkorn, Ulrike
AU - Steinhoff, Ulrich
PY - 2006
Y1 - 2006
N2 - Microbial infections induce the replacement of constitutive proteasomes by immunoproteasomes (I-proteasomes). I-proteasomes support efficient generation of MHC class I epitopes and influence immunodominance hierarchies of CD8(+) T cells. Recently, the function of I-proteasomes in antimicrobial responses was challenged by showing that the lack of I-proteasomes has no effect on induction and function of lymphocytic choriomeningitis virus-specific CD8(+) T cells. Here, we show that infection with Listeria monocytogenes rapidly induces I-proteasomes in nonlymphoid tissues, which leads to enhanced generation of protection relevant CD8(+) T cell epitopes. I-proteasome-deficient mice (beta 5i(-/-) mice) exhibited normal frequencies of L monocytogenes-specific CD8(+) T cells. However, clearance of L. monocytogenes in liver but not spleen was significantly impaired in I-proteasome-deficient mice. In summary, our studies demonstrate that induction of I-proteasomes is required for CD8(+) T cell-mediated elimination of L monocytogenes from nonlymphoid but not lymphoid tissues.
AB - Microbial infections induce the replacement of constitutive proteasomes by immunoproteasomes (I-proteasomes). I-proteasomes support efficient generation of MHC class I epitopes and influence immunodominance hierarchies of CD8(+) T cells. Recently, the function of I-proteasomes in antimicrobial responses was challenged by showing that the lack of I-proteasomes has no effect on induction and function of lymphocytic choriomeningitis virus-specific CD8(+) T cells. Here, we show that infection with Listeria monocytogenes rapidly induces I-proteasomes in nonlymphoid tissues, which leads to enhanced generation of protection relevant CD8(+) T cell epitopes. I-proteasome-deficient mice (beta 5i(-/-) mice) exhibited normal frequencies of L monocytogenes-specific CD8(+) T cells. However, clearance of L. monocytogenes in liver but not spleen was significantly impaired in I-proteasome-deficient mice. In summary, our studies demonstrate that induction of I-proteasomes is required for CD8(+) T cell-mediated elimination of L monocytogenes from nonlymphoid but not lymphoid tissues.
M3 - Journal article
VL - 177
SP - 6238
EP - 6244
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -