Immunoprophylaxis in fish by injection of mouse antibody genes

Niels Lorenzen; P.M. Cupit; Katja Einer-Jensen; Ellen Lorenzen; Peter Ahrens; C.J. Secombes; C. Cunningham

doi:10.1038/81169

Immunoprophylaxis in fish by injection of mouse antibody genes

Niels Lorenzen
, P.M. Cupit
, Katja Einer-Jensen
, Ellen Lorenzen
, Peter Ahrens
, C.J. Secombes
, C. Cunningham

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

Original language	English
Journal	Nature Biotechnology
Volume	18
Issue number	11
Pages (from-to)	1177-1180
ISSN	1087-0156
DOIs	https://doi.org/10.1038/81169
Publication status	Published - 2000

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

gene therapy
rhabdovirus
single-chain antibody

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10.1038/81169

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title = "Immunoprophylaxis in fish by injection of mouse antibody genes",

abstract = "Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.",

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AU - Lorenzen, Niels

AU - Cupit, P.M.

AU - Einer-Jensen, Katja

AU - Lorenzen, Ellen

AU - Ahrens, Peter

AU - Secombes, C.J.

AU - Cunningham, C.

PY - 2000

Y1 - 2000

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AB - Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

KW - gene therapy

KW - rhabdovirus

KW - single-chain antibody

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IS - 11

ER -

Immunoprophylaxis in fish by injection of mouse antibody genes

Abstract

UN SDGs

Keywords

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