Immunoprophylaxis in fish by injection of mouse antibody genes

Niels Lorenzen, P.M. Cupit, Katja Einer-Jensen, Ellen Lorenzen, Peter Ahrens, C.J. Secombes, C. Cunningham

    Research output: Contribution to journalJournal articlepeer-review

    Abstract

    Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.
    Original languageEnglish
    JournalNature Biotechnology
    Volume18
    Issue number11
    Pages (from-to)1177-1180
    ISSN1087-0156
    DOIs
    Publication statusPublished - 2000

    Keywords

    • gene therapy
    • rhabdovirus
    • single-chain antibody

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