Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

Joan Klausen; V. Perez; Steen Bjørck Giese; J.F.G. Marin; Peter Ahrens

doi:10.1016/S0378-1135(97)00133-8

Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

Joan Klausen, V. Perez, Steen Bjørck Giese, J.F.G. Marin, Peter Ahrens

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.

Original language	English
Journal	Veterinary Microbiology
Volume	51
Issue number	2-3
Pages (from-to)	181-187
ISSN	0378-1135
DOIs	https://doi.org/10.1016/S0378-1135(97)00133-8
Publication status	Published - 1997

Keywords

immunology
Mycobacterium avium
ELISA

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10.1016/S0378-1135(97)00133-8

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title = "Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein",

abstract = "A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.",

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author = "Joan Klausen and V. Perez and Giese, {Steen Bj{\o}rck} and J.F.G. Marin and Peter Ahrens",

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journal = "Veterinary Microbiology",

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Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein. / Klausen, Joan; Perez, V.; Giese, Steen Bjørck et al.
In: Veterinary Microbiology, Vol. 51, No. 2-3, 1997, p. 181-187.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

AU - Klausen, Joan

AU - Perez, V.

AU - Giese, Steen Bjørck

AU - Marin, J.F.G.

AU - Ahrens, Peter

PY - 1997

Y1 - 1997

N2 - A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.

AB - A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.

KW - immunology

KW - Mycobacterium avium

KW - ELISA

U2 - 10.1016/S0378-1135(97)00133-8

DO - 10.1016/S0378-1135(97)00133-8

M3 - Journal article

SN - 0378-1135

VL - 51

SP - 181

EP - 187

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 2-3

ER -

Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

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