Immunohistochemical investigation of the tissue distribution of mannan-binding lectin in non-infected and virus-infected chickens

O.L. Nielsen, Poul Henrik Jørgensen, J. Hedemand, J.C. Jensenius, C. Koch, S.B. Laursen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This-paper describes the results of immuno-histochemical staining for chicken mannan-binding lectin (MBL) in formalin-fixed tissue sections from non-infected chickens, and from chickens infected with infectious laryngotracheitis virus (ILTV) or infectious bursal disease virus (IBDV). In the non-infected chickens, MBL was detected in the cytoplasm of a few hepatocytes and in the germinal centres of the caecal tonsils, whereas sections of kidney, heart muscle, spleen, cerebrum, thymus, adrenal gland, bursa of Fabricius, bone marrow and trachea were without staining. In the ILTV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes and on the surface of, and inside, ILTV-infected cells. Also in the IBDV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes. No staining was seen in the follicles of the bursa of Fabricius, but MBL was present in non-identified cells in the interstitium, and in the cytoplasm of macrophage-like cells, located peripheral to the ellipsoid of the spleen. These findings indicate the liver as the primary site of MBL synthesis, and points to up-regulation as a result of the viral infections. The location outside the liver could indicate a role of MBL in the immune defence.
    Original languageEnglish
    JournalImmunology
    Volume94
    Issue number1
    Pages (from-to)122-128
    ISSN0019-2805
    DOIs
    Publication statusPublished - 1998

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