Immunohistochemical detection of interleukin-8 in inflamed porcine tissues

Henriette Laursen, Henrik Elkær Jensen, Páll S. Leifsson, Louise K. Jensen, Johanna G. Christiansen, Ramona Trebbien, Ole L. Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion. Furthermore, endothelial cells of newly formed vessels in granulation tissue were positive in some specimens. Some sub-populations of inflammatory neutrophils were, however, IL-8 negative which could reflect some phase of neutrophil swarming.
    Original languageEnglish
    JournalVeterinary Immunology and Immunopathology
    Volume159
    Pages (from-to)97-102
    Number of pages6
    ISSN0165-2427
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Interleukin-8
    • Pigs
    • Immunohistochemistry
    • Neutrophils
    • Inflammation
    • Swarming

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