Immunisation against PCV2 structural protein by DNA vaccination of mice

Søren Kamstrup, Annette Malene Barfoed, Tine Frimann, Anne-Sofie Ladekjær-Mikkelsen, Anette Bøtner

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome (PMWS). The disease affects primarily 5-12-weeks-old pigs which might suggest that infection with PCV2 occurs when the level of maternal antibodies have declined to sub-protective levels around weaning at 3-5-weeks of age. If immunoprophylaxis is to be effective, an immunisation method capable of breaking through maternal immunity must be employed. In this study, we have developed and investigated the potential of a DNA vaccination approach to be one such method. The gene encoding the capsid protein of PCV2 was cloned in a DNA vaccination plasmid and expression of capsid protein was demonstrated in vitro. Mice were gene gun vaccinated three timesand all mice responded serologically by raising antibodies against PCV2. The results suggest, that DNA based vaccination might offer opportunities for vaccination of piglets against PCV2.
    Original languageEnglish
    JournalVaccine
    Volume22
    Issue number11-12
    Pages (from-to)1358-1361
    ISSN0264-410X
    DOIs
    Publication statusPublished - 2004

    Keywords

    • immunoprophylaxis
    • porcine circovirus type 2
    • postweaning multisystemic wasting syndrome

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