Immobilization of L-arabinitol dehydrogenase on aldehyde-functionalized silicon oxide nanoparticles for L-xylulose production

Raushan Kumar Singh, Manish Kumar Tiwari, Ranjitha Singh, Jung-Rim Haw, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review


l-Xylulose is a potential starting material for therapeutics. However, its translation into clinical practice has been hampered by its inherently low bioavailability. In addition, the high cost associated with the production of l-xylulose is a major factor hindering its rapid deployment beyond the laboratory. In the current study, l-arabinitol 4-dehydrogenase from Hypocrea jecorina (HjLAD), which catalyzes the conversion of l-arabinitol into l-xylulose, was immobilized onto various carriers, and the immobilized enzymes were characterized. HjLAD covalently immobilized onto silicon oxide nanoparticles showed the highest immobilization efficiency (94.7 %). This report presents a comparative characterization of free and immobilized HjLAD, including its thermostability and kinetic parameters. The thermostability of HjLAD immobilized on silicon oxide nanoparticles was more than 14.2-fold higher than free HjLAD; the t(1/2) of HjLAD at 25 A degrees C was enhanced from 190 min (free) to 45 h (immobilized). In addition, the immobilized HjLAD retained 94 % of its initial activity after 10 cycles. When the immobilized HjLAD was used to catalyze the biotransformation of l-arabinitol to l-xylulose, 66 % conversion and a productivity of 7.9 g A center dot h(-1) A center dot L-1 were achieved. The enhanced thermostability and reusability of HjLAD suggest that immobilization of HjLAD onto silicon oxide nanoparticles has the potential for use in the industrial production of rare sugars.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Issue number3
Pages (from-to)1095-1104
Publication statusPublished - 2014
Externally publishedYes


  • enzyme activity
  • thermostability
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Hypocrea jecorina species
  • glutaraldehyde 111-30-8
  • L-arabinitol 4-dehydrogenase EC
  • L-arabinitol 7643-75-6
  • L-xylulose 527-50-4
  • 10060, Biochemistry studies - General
  • 10511, Biophysics - Bioengineering
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 39008, Food microbiology - General and miscellaneous
  • 51518, Plant physiology - Enzymes
  • Biochemistry and Molecular Biophysics
  • aldehyde-functionalized silicon oxide nanoparticle
  • Biomaterials
  • Bioprocess Engineering
  • Enzymology
  • L-Arabinitol 4-dehydrogenase
  • Immobilization
  • Silicon oxide nanoparticles
  • MWCNTs
  • L-Xylulose
  • l-Arabinitol 4-dehydrogenase
  • l-Xylulose
  • Biochemistry
  • Nanoparticles
  • Radioactive waste vitrification
  • Reusability
  • Stability
  • Comparative characterizations
  • Immobilization efficiency
  • Immobilized enzyme
  • Industrial production
  • Silicon oxides
  • HASH(0x4c1edd0)

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