Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

Maria Godoy Gallardo, Núria Portolés-Gil, Ana M. López-Periago, Concepción Domingo, Leticia Hosta-Rigau*

*Corresponding author for this work

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Abstract

We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.
Original languageEnglish
Article number6418
JournalInternational Journal of Molecular Sciences
Volume21
Issue number17
Number of pages25
ISSN1661-6596
DOIs
Publication statusPublished - 2020

Keywords

  • Angiogenesis
  • Bone tissue engineering
  • Co-delivery
  • Growth factors
  • Multilayers
  • Osteogenesis
  • Polydopamine
  • Scaffolds

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