Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy, Jonathan Nylk, Federico M. Gasparoli, Adrià Escobet-Montalbán, Anders K. Hansen, Dominik Marti, Peter E. Andersen, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Abstract

Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
PublisherSPIE - International Society for Optical Engineering
Publication date2020
DOIs
Publication statusPublished - 2020
EventSPIE Photonics West 2020: SPIE BIOS: Adaptive Optics and Wavefront Control for Biological Systems VI - The Moscone Center, San Francisco, United States
Duration: 1 Feb 20206 Feb 2020
Conference number: 11248
https://spie.org/conferences-and-exhibitions/photonics-west/bios

Conference

ConferenceSPIE Photonics West 2020: SPIE BIOS
Number11248
LocationThe Moscone Center
CountryUnited States
CitySan Francisco
Period01/02/202006/02/2020
Internet address
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume11245
ISSN0361-0748

Cite this

Veettikazhy, M., Nylk, J., Gasparoli, F. M., Escobet-Montalbán, A., Hansen, A. K., Marti, D., Andersen, P. E., & Dholakia, K. (2020). Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII SPIE - International Society for Optical Engineering. Proceedings of SPIE, the International Society for Optical Engineering, Vol.. 11245 https://doi.org/10.1117/12.2544205