Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy; Jonathan  Nylk; Federico M.  Gasparoli; Adrià  Escobet-Montalbán; Anders K. Hansen; Dominik Marti; Peter E. Andersen; Kishan Dholakia

doi:10.1117/12.2544205

Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy, Jonathan Nylk, Federico M. Gasparoli, Adrià Escobet-Montalbán, Anders K. Hansen, Dominik Marti, Peter E. Andersen, Kishan Dholakia

Department of Health Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

Abstract

Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Publisher	SPIE - International Society for Optical Engineering
Publication date	2020
DOIs	https://doi.org/10.1117/12.2544205
Publication status	Published - 2020
Event	SPIE Photonics West BiOS 2020 - The Moscone Center, San Francisco, United States Duration: 1 Feb 2020 → 6 Feb 2020 https://spie.org/conferences-and-exhibitions/photonics-west/bios

Conference

Conference	SPIE Photonics West BiOS 2020
Location	The Moscone Center
Country/Territory	United States
City	San Francisco
Period	01/02/2020 → 06/02/2020
Sponsor	SPIE
Internet address	https://spie.org/conferences-and-exhibitions/photonics-west/bios

Series	Proceedings of SPIE, the International Society for Optical Engineering
Volume	11245
ISSN	0361-0748

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Veettikazhy, M., Nylk, J., Gasparoli, F. M., Escobet-Montalbán, A., Hansen, A. K., Marti, D., Andersen, P. E., & Dholakia, K. (2020). Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII SPIE - International Society for Optical Engineering. https://doi.org/10.1117/12.2544205

Veettikazhy, Madhu ; Nylk, Jonathan ; Gasparoli, Federico M. et al. / Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII. SPIE - International Society for Optical Engineering, 2020. (Proceedings of SPIE, the International Society for Optical Engineering, Vol. 11245).

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title = "Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)",

abstract = "Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A {\textquoteleft}gold standard{\textquoteright} system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.",

author = "Madhu Veettikazhy and Jonathan Nylk and Gasparoli, {Federico M.} and Adri{\`a} Escobet-Montalb{\'a}n and Hansen, {Anders K.} and Dominik Marti and Andersen, {Peter E.} and Kishan Dholakia",

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doi = "10.1117/12.2544205",

language = "English",

series = "Proceedings of SPIE, the International Society for Optical Engineering",

booktitle = "Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII",

publisher = "SPIE - International Society for Optical Engineering",

note = "SPIE Photonics West BiOS 2020 ; Conference date: 01-02-2020 Through 06-02-2020",

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Veettikazhy, M, Nylk, J, Gasparoli, FM, Escobet-Montalbán, A, Hansen, AK, Marti, D, Andersen, PE & Dholakia, K 2020, Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII. SPIE - International Society for Optical Engineering, Proceedings of SPIE, the International Society for Optical Engineering, vol. 11245, SPIE Photonics West BiOS 2020, San Francisco, California, United States, 01/02/2020. https://doi.org/10.1117/12.2544205

Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). / Veettikazhy, Madhu; Nylk, Jonathan ; Gasparoli, Federico M. et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII. SPIE - International Society for Optical Engineering, 2020. (Proceedings of SPIE, the International Society for Optical Engineering, Vol. 11245).

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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AU - Veettikazhy, Madhu

AU - Nylk, Jonathan

AU - Gasparoli, Federico M.

AU - Escobet-Montalbán, Adrià

AU - Hansen, Anders K.

AU - Marti, Dominik

AU - Andersen, Peter E.

AU - Dholakia, Kishan

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AB - Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.

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M3 - Conference abstract in proceedings

T3 - Proceedings of SPIE, the International Society for Optical Engineering

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Veettikazhy M, Nylk J, Gasparoli FM, Escobet-Montalbán A, Hansen AK, Marti D et al. Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation). In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII. SPIE - International Society for Optical Engineering. 2020. (Proceedings of SPIE, the International Society for Optical Engineering, Vol. 11245). doi: 10.1117/12.2544205

Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

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