Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth (Conference Presentation)

Madhu Veettikazhy, Jonathan Nylk, Federico M. Gasparoli, Adrià Escobet-Montalbán, Anders K. Hansen, Dominik Marti, Peter E. Andersen, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review


Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
PublisherSPIE - International Society for Optical Engineering
Publication date2020
Publication statusPublished - 2020
EventSPIE Photonics West 2020: SPIE BIOS: Adaptive Optics and Wavefront Control for Biological Systems VI - The Moscone Center, San Francisco, United States
Duration: 1 Feb 20206 Feb 2020
Conference number: 11248


ConferenceSPIE Photonics West 2020: SPIE BIOS
LocationThe Moscone Center
CountryUnited States
CitySan Francisco
Internet address
SeriesProceedings of SPIE, the International Society for Optical Engineering

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