Imaging regional metabolic changes in the ischemic rat heart in vivo using hyperpolarized(1-13C)Pyruvate

Mette Hauge Lauritzen, Peter Magnusson, Christoffer Laustsen, Sadia Asghar Butt, Jan Henrik Ardenkjær-Larsen, Lise Vejby Søgaard, Olaf B. Paulson, Per Åkeson

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    Abstract

    We evaluated the use of hyperpolarized 13C magnetic resonance imaging (MRI) in an open-chest rat model of myocardial infarction to image regional changes in myocardial metabolism. In total, 10 rats were examined before and after 30 minutes of occlusion of the left anterior descending coronary artery using hyperpolarized [1-13C]pyruvate. Cardiac metabolic images of [1-13C]pyruvate and its metabolites [1-13C]lactate, [1-13C]alanine, and [13C]bicarbonate were obtained before and after ischemia. Significant reduction in the [1-13C]alanine and [1-13C]lactate signals were observed in the ischemic region post ischemia. The severity of the ischemic insult was verified by increased blood levels of troponin I and by using late contrast-enhanced MRI that showed enhanced signal in the ischemic region. This study shows that hyperpolarized MRI can be used to image regional metabolic changes in the in vivo rat heart in an open-chest model of ischemia reperfusion. Hyperpolarized MRI enables new possibilities for evaluating changes in cardiac metabolism noninvasively and in real time, which potentially could be used for research to evaluate new treatments and metabolic interventions for myocardial ischemia and to apply knowledge to future application of the technique in humans.
    Original languageEnglish
    JournalTomography
    Volume3
    Issue number3
    Pages (from-to)123-130
    ISSN2379-1381
    DOIs
    Publication statusPublished - 2017

    Bibliographical note

    This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

    Keywords

    • Magnetic Resonance Imaging
    • Cardiac metabolism
    • Ischemic heart disease
    • Myocardial infarction
    • Hyperpolarized 13C pyruvate
    • Rat model

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