Imaging of Gadolinium Spatial Distribution in Tumor Tissue by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Nazila Kamaly, John A. Pugh, T. L. Kalber, Josephine Bunch, Andrew D. Miller, Cameron W. McLeod, Jimmy Bell

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Purpose
Laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS) was utilized in postmortem imaging of gadolinium (Gd) spatial distribution in a mouse tumor model postadministration of PEGylated Gd liposomal nanoparticles.
Procedures
PEGylated liposomal nanoparticles were formulated using a paramagnetic lipid incorporating Gd, in addition to a fluorescent lipid, and injected intravenously into Balb/C nude mice bearing IGROV-1 tumors. At postinjection (2 h), the tumors and selective organs were imaged by magnetic resonance imaging (MRI) and, after excision, by histology and LA–ICP–MS.
Results
The presence of Gd within tumor tissue was confirmed by LA–ICP–MS and when correlated to histology was found to be prevalent in regions of higher vascularity. The presence of Gd in the kidneys was also confirmed.
Conclusions
We have demonstrated, in a novel manner, the use of LA–ICP–MS for the spatial detection of Gd in tumor tissue. LA–ICP–MS is valuable in providing spatio-specific information of MRI contrast agents and more importantly Gd in tumor tissue.
Original languageEnglish
JournalMolecular Imaging and Biology
Volume12
Issue number4
Pages (from-to)361–366
ISSN1536-1632
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • LA–ICP–MS
  • Gd contrast agents
  • Tumor MRI
  • Gd biodistribution
  • Multimodal imaging
  • Liposomes
  • Nanoparticles
  • Fluorescence imaging

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