Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.

Sadia Asghar Butt; Lise Vejby-Christensen Søgaard; Peter O. Magnusson; Mette Hauge Lauritzen; Christoffer Laustsen; Per Akeson; Jan H Ardenkjær-Larsen

doi:10.1038/jcbfm.2012.34

Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.

Sadia Asghar Butt
, Lise Vejby-Christensen Søgaard
, Peter O. Magnusson
, Mette Hauge Lauritzen
, Christoffer Laustsen
, Per Akeson
, Jan H Ardenkjær-Larsen

Copenhagen University Hospital Herlev and Gentofte

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow & Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.

Original language	English
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	32
Issue number	8
Pages (from-to)	1508-1514
ISSN	0271-678X
DOIs	https://doi.org/10.1038/jcbfm.2012.34
Publication status	Published - 2012

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@article{247a44bdef5b49a9a55c6bc419adc7e4,

title = "Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.",

abstract = "The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow \& Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.",

author = "Butt, \{Sadia Asghar\} and S{\o}gaard, \{Lise Vejby-Christensen\} and Magnusson, \{Peter O.\} and Lauritzen, \{Mette Hauge\} and Christoffer Laustsen and Per Akeson and Ardenkj{\ae}r-Larsen, \{Jan H\}",

year = "2012",

doi = "10.1038/jcbfm.2012.34",

language = "English",

volume = "32",

pages = "1508--1514",

journal = "Journal of Cerebral Blood Flow and Metabolism",

issn = "0271-678X",

publisher = "SAGE Publications Inc.",

number = "8",

}

Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging. / Butt, Sadia Asghar; Søgaard, Lise Vejby-Christensen; Magnusson, Peter O. et al.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 32, No. 8, 2012, p. 1508-1514.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.

AU - Butt, Sadia Asghar

AU - Søgaard, Lise Vejby-Christensen

AU - Magnusson, Peter O.

AU - Lauritzen, Mette Hauge

AU - Laustsen, Christoffer

AU - Akeson, Per

AU - Ardenkjær-Larsen, Jan H

PY - 2012

Y1 - 2012

N2 - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow & Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.

AB - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.Journal of Cerebral Blood Flow & Metabolism advance online publication, 28 March 2012; doi:10.1038/jcbfm.2012.34.

U2 - 10.1038/jcbfm.2012.34

DO - 10.1038/jcbfm.2012.34

M3 - Journal article

C2 - 22453630

SN - 0271-678X

VL - 32

SP - 1508

EP - 1514

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

IS - 8

ER -

Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C Magnetic Resonance Spectroscopic Imaging.

Abstract

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