Illuminating the binding interactions between the JJ1 inhibitor and α-cobratoxin by HDX-MS

Henrik Flindt; Tam T. T. N. Nguyen; Andreas Hougaard Laustsen; Brian Lohse; Kasper Rand

Illuminating the binding interactions between the JJ1 inhibitor and α-cobratoxin by HDX-MS

Henrik Flindt
, Tam T. T. N. Nguyen
, Andreas Hougaard Laustsen
, Brian Lohse
, Kasper Rand

University of Copenhagen

Research output: Contribution to conference › Poster › Research › peer-review

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Abstract

Snakebite is a constant public health concern in the tropical parts of the world with antivenom being the only effective therapy. Despite their essential role in snakebite envenoming therapy, antivenoms are associated with severe side effects due to their heterologous nature. To overcome these problems, a new approach is needed. Based on phage display selection, a peptidic inhibitor (JJ1) was discovered, which targets the potent α-cobratoxin from Naja kaouthia. Here, we report our efforts on characterizing the interactions between JJ1 and α-cobratoxin by H/D exchange mass spectrometry (HDX-MS).

Original language	English
Publication date	2016
Number of pages	1
Publication status	Published - 2016
Externally published	Yes
Event	14th Protein.DTU Workshop: Applied Protein Science - Technical University of denmark, Kgs. Lyngby, Denmark Duration: 9 May 2016 → 9 May 2016 Conference number: 14

Workshop

Workshop	14th Protein.DTU Workshop
Number	14
Location	Technical University of denmark
Country/Territory	Denmark
City	Kgs. Lyngby
Period	09/05/2016 → 09/05/2016

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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@conference{9e1a3b2b104540d899fe1787f1df8edc,

title = "Illuminating the binding interactions between the JJ1 inhibitor and α-cobratoxin by HDX-MS",

abstract = "Snakebite is a constant public health concern in the tropical parts of the world with antivenom being the only effective therapy. Despite their essential role in snakebite envenoming therapy, antivenoms are associated with severe side effects due to their heterologous nature. To overcome these problems, a new approach is needed. Based on phage display selection, a peptidic inhibitor (JJ1) was discovered, which targets the potent α-cobratoxin from Naja kaouthia. Here, we report our efforts on characterizing the interactions between JJ1 and α-cobratoxin by H/D exchange mass spectrometry (HDX-MS).",

author = "Henrik Flindt and Nguyen, \{Tam T. T. N.\} and Laustsen, \{Andreas Hougaard\} and Brian Lohse and Kasper Rand",

year = "2016",

language = "English",

note = "14th Protein.DTU Workshop : Applied Protein Science ; Conference date: 09-05-2016 Through 09-05-2016",

}

TY - CONF

T1 - Illuminating the binding interactions between the JJ1 inhibitor and α-cobratoxin by HDX-MS

AU - Flindt, Henrik

AU - Nguyen, Tam T. T. N.

AU - Laustsen, Andreas Hougaard

AU - Lohse, Brian

AU - Rand, Kasper

N1 - Conference code: 14

PY - 2016

Y1 - 2016

N2 - Snakebite is a constant public health concern in the tropical parts of the world with antivenom being the only effective therapy. Despite their essential role in snakebite envenoming therapy, antivenoms are associated with severe side effects due to their heterologous nature. To overcome these problems, a new approach is needed. Based on phage display selection, a peptidic inhibitor (JJ1) was discovered, which targets the potent α-cobratoxin from Naja kaouthia. Here, we report our efforts on characterizing the interactions between JJ1 and α-cobratoxin by H/D exchange mass spectrometry (HDX-MS).

AB - Snakebite is a constant public health concern in the tropical parts of the world with antivenom being the only effective therapy. Despite their essential role in snakebite envenoming therapy, antivenoms are associated with severe side effects due to their heterologous nature. To overcome these problems, a new approach is needed. Based on phage display selection, a peptidic inhibitor (JJ1) was discovered, which targets the potent α-cobratoxin from Naja kaouthia. Here, we report our efforts on characterizing the interactions between JJ1 and α-cobratoxin by H/D exchange mass spectrometry (HDX-MS).

M3 - Poster

T2 - 14th Protein.DTU Workshop

Y2 - 9 May 2016 through 9 May 2016

ER -

Illuminating the binding interactions between the JJ1 inhibitor and α-cobratoxin by HDX-MS

Abstract

Workshop

UN SDGs

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