Identification of the Entner-Doudoroff pathway in an antibiotic-producing actinomycete species

Nina Gunnarsson, Uffe Hasbro Mortensen, M. Sosio, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review


    The metabolic network of the central carbon metabolism represents the backbone of cellular metabolism and provides the precursors and cofactors required for synthesis of secondary metabolites. It is therefore pivotal to map the operating metabolic network in the central carbon metabolism in order to design metabolic engineering strategies towards construction of more efficient producers of specific metabolites. In this context, methods that allow rapid and reliable mapping of the central carbon metabolism are valuable. In the present study, a C-13 labelling-based method was used to identify the primary metabolic pathways of the poorly characterized antibiotic-producing actinomycete Nonomuraea sp. ATCC 39727. Surprisingly, it was found that Nonomuraea sp. ATCC 39272 predominantly metabolizes glucose via the Entner-Doudoroff (ED) pathway. This represents the first time that the ED pathway has been recognized as the main catabolic pathway in an actinomycete. The Nonomuraea genes encoding the key enzymes of the ED pathway were subsequently identified, sequenced and functionally described.
    Original languageEnglish
    JournalMolecular Microbiology
    Issue number3
    Pages (from-to)895-902
    Publication statusPublished - 2004


    Dive into the research topics of 'Identification of the Entner-Doudoroff pathway in an antibiotic-producing actinomycete species'. Together they form a unique fingerprint.

    Cite this