Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

Stephen L.W. Ona; Clare S. Harringtona

doi:10.1111/j.1574-6968.2000.tb09419.x

Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

Stephen L.W. Ona, Clare S. Harringtona

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Amplified fragment length polymorphism (AFLP)-based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S-) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter. hyointestinalis, and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S-levels. All outbreak or 'genetically identical' strains of C. jejuni subsp. jejuni, Campylobacter coli, C. hyointestinalis and C. sputorum clustered at S-levels > 92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.

Original language	English
Journal	FEMS Microbiology Letters
Volume	193
Issue number	1
Pages (from-to)	161-169
ISSN	0378-1097
DOIs	https://doi.org/10.1111/j.1574-6968.2000.tb09419.x
Publication status	Published - 2000

Keywords

epidemiological typing
AFLP
identification
Campylobacter
taxonomy

Access to Document

10.1111/j.1574-6968.2000.tb09419.x

Fingerprint Dive into the research topics of 'Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles'. Together they form a unique fingerprint.

Cite this

@article{03265a530ff843419e09e4ee0eb8667f,

title = "Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles",

abstract = "Amplified fragment length polymorphism (AFLP)-based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S-) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter. hyointestinalis, and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S-levels. All outbreak or 'genetically identical' strains of C. jejuni subsp. jejuni, Campylobacter coli, C. hyointestinalis and C. sputorum clustered at S-levels > 92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.",

keywords = "epidemiological typing, AFLP, identification, Campylobacter, taxonomy",

author = "Ona, {Stephen L.W.} and Harringtona, {Clare S.}",

year = "2000",

doi = "10.1111/j.1574-6968.2000.tb09419.x",

language = "English",

volume = "193",

pages = "161--169",

journal = "F E M S Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

AU - Ona, Stephen L.W.

AU - Harringtona, Clare S.

PY - 2000

Y1 - 2000

N2 - Amplified fragment length polymorphism (AFLP)-based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S-) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter. hyointestinalis, and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S-levels. All outbreak or 'genetically identical' strains of C. jejuni subsp. jejuni, Campylobacter coli, C. hyointestinalis and C. sputorum clustered at S-levels > 92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.

AB - Amplified fragment length polymorphism (AFLP)-based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S-) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter. hyointestinalis, and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S-levels. All outbreak or 'genetically identical' strains of C. jejuni subsp. jejuni, Campylobacter coli, C. hyointestinalis and C. sputorum clustered at S-levels > 92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.

KW - epidemiological typing

KW - AFLP

KW - identification

KW - Campylobacter

KW - taxonomy

U2 - 10.1111/j.1574-6968.2000.tb09419.x

DO - 10.1111/j.1574-6968.2000.tb09419.x

M3 - Journal article

VL - 193

SP - 161

EP - 169

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 1

ER -