Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

Stephen L.W. Ona, Clare S. Harringtona

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Amplified fragment length polymorphism (AFLP)-based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S-) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter. hyointestinalis, and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S-levels. All outbreak or 'genetically identical' strains of C. jejuni subsp. jejuni, Campylobacter coli, C. hyointestinalis and C. sputorum clustered at S-levels > 92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume193
    Issue number1
    Pages (from-to)161-169
    ISSN0378-1097
    DOIs
    Publication statusPublished - 2000

    Keywords

    • epidemiological typing
    • AFLP
    • identification
    • Campylobacter
    • taxonomy

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