Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets

Lasse Eggers Pedersen, Solvej Østergaard Breum, Ulla Riber, Lars Erik Larsen, Gregers Jungersen

    Research output: Contribution to journalJournal articleResearchpeer-review

    291 Downloads (Pure)

    Abstract

    Background: Major histocompatibility complex (MHC) class I peptide binding and presentation are essential for antigen-specific activation of cytotoxic T lymphocytes (CTLs) and swine MHC class I molecules, also termed swine leukocyte antigens (SLA), thus play a crucial role in the process that leads to elimination of viruses such as swine influenza virus (SwIV). This study describes the identification of SLA-presented peptide epitopes that are targets for a swine CTL response, and further analyses multiple specificities expressed by SwIV activated CTL subsets. Findings: Four SwIV derived peptides were identified as T cell epitopes using fluorescent influenza: SLA tetramers. In addition, multiple CTL specificities were analyzed using peptide sequence substitutions in two of the four epitope candidates analyzed. Interestingly both conserved and substituted peptides were found to stain the CD4-CD8+ T cell subsets indicating multiple specificities. Conclusions: This study describes a timely and cost-effective approach for viral epitope identification in livestock animals. Analysis of T cell subsets showed multiple specificities suggesting SLA-bound epitope recognition of different conformations.
    Original languageEnglish
    JournalVirology Journal
    Volume11
    Issue number163
    Number of pages5
    ISSN1743-422X
    DOIs
    Publication statusPublished - 2014

    Keywords

    • VIROLOGY
    • CROSS-REACTIVITY
    • PEPTIDE-BINDING
    • MHC
    • SEQUENCE
    • PIGS
    • LYMPHOCYTES
    • PREDICTION
    • NETMHCPAN
    • TETRAMERS
    • PROTEIN
    • Swine influenza virus
    • Major histocompatibility complex
    • Cytotoxic T cell
    • Viral epitope

    Fingerprint Dive into the research topics of 'Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets'. Together they form a unique fingerprint.

    Cite this