Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

Melina Fischer; Conrad M. Freuling; Thomas Müller; Juliane Schatz; Thomas Bruun Rasmussen; Mariann Chriél; Anne Balkema-Buschmann; Martin Beer; Bernd Hoffmann

doi:10.1186/s12985-014-0196-x

Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

Melina Fischer, Conrad M. Freuling, Thomas Müller, Juliane Schatz, Thomas Bruun Rasmussen, Mariann Chriél, Anne Balkema-Buschmann, Martin Beer, Bernd Hoffmann

Friedrich-Loeffler-Institute

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.

Original language	English
Journal	The Open Virology Journal
Volume	11
Issue number	196
Number of pages	4
ISSN	1874-3579
DOIs	https://doi.org/10.1186/s12985-014-0196-x
Publication status	Published - 2014

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title = "Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats",

abstract = "Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.",

author = "Melina Fischer and Freuling, \{Conrad M.\} and Thomas M{\"u}ller and Juliane Schatz and Rasmussen, \{Thomas Bruun\} and Mariann Chri{\'e}l and Anne Balkema-Buschmann and Martin Beer and Bernd Hoffmann",

year = "2014",

doi = "10.1186/s12985-014-0196-x",

language = "English",

volume = "11",

journal = "The Open Virology Journal",

issn = "1874-3579",

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number = "196",

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TY - JOUR

T1 - Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

AU - Fischer, Melina

AU - Freuling, Conrad M.

AU - Müller, Thomas

AU - Schatz, Juliane

AU - Rasmussen, Thomas Bruun

AU - Chriél, Mariann

AU - Balkema-Buschmann, Anne

AU - Beer, Martin

AU - Hoffmann, Bernd

PY - 2014

Y1 - 2014

N2 - Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.

AB - Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.

U2 - 10.1186/s12985-014-0196-x

DO - 10.1186/s12985-014-0196-x

M3 - Journal article

C2 - 25420461

SN - 1874-3579

VL - 11

JO - The Open Virology Journal

JF - The Open Virology Journal

IS - 196

ER -

Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

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