Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

Melina Fischer, Conrad M. Freuling, Thomas Müller, Juliane Schatz, Thomas Bruun Rasmussen, Mariann Chriél, Anne Balkema-Buschmann, Martin Beer, Bernd Hoffmann

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    Abstract

    Background: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).Findings: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
    Original languageEnglish
    JournalThe Open Virology Journal
    Volume11
    Issue number196
    Number of pages4
    ISSN1874-3579
    DOIs
    Publication statusPublished - 2014

    Cite this

    Fischer, M., Freuling, C. M., Müller, T., Schatz, J., Rasmussen, T. B., Chriél, M., Balkema-Buschmann, A., Beer, M., & Hoffmann, B. (2014). Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats. The Open Virology Journal, 11(196). https://doi.org/10.1186/s12985-014-0196-x